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  • Food Safety
    • Recalls & Public Health Alerts
      • Report a Problem with Food
        • Additional Recalls
      • Annual Recall Summaries
        • Summary of Recall and PHA Cases in Calendar Year 2023
        • Summary of Recall and PHA Cases in Calendar Year 2022
        • Summary of Recall Cases in Calendar Year 2021
        • Summary of Recall Cases in Calendar Year 2020
        • Summary of Recall Cases in Calendar Year 2019
        • Summary of Recall Cases in Calendar Year 2018
        • Summary of Recall Cases in Calendar Year 2017
        • Summary of Recall Cases in Calendar Year 2016
        • Summary of Recall Cases in Calendar Year 2015
        • Summary of Recall Cases in Calendar Year 2014
        • Summary of Recall Cases in Calendar Year 2013
        • Summary of Recall Cases in Calendar Year 2012
    • Food Safety Stats
      • Consumer Research
    • Foodborne Illness and Disease
      • Enfermedades Transmitidas Por Alimentos y Otras Enfermedades
      • Illnesses and Pathogens
        • Botulism
          • Clostridium botulinum y El Botulismo
        • Campylobacter
          • Campylobacter En Español
        • E. coli
        • Listeria
        • Parasites and Foodborne Illness
          • Parásitos y Enfermedades Transmitidas Por los Alimentos
        • Preguntas y Respuestas Sobre Listeria
        • Salmonella
          • Preguntas y Respuestas Sobre Salmonella
      • Resources for Public Health Partners
        • State Departments of Public Health
      • Outbreaks
        • Outbreak Investigations: Prevention
        • Outbreak Investigations: Response
    • Safe Food Handling and Preparation
      • Food Safety Basics
        • Additives in Meat and Poultry Products
        • Aditivos en Productos Cárnicos y Avícolas
        • Agua en Carnes y Aves
        • Ahumar Carnes y Aves
        • Air Fryers and Food Safety
        • Alergias Alimentarias: Los “9 Grandes”
        • Appliance Thermometers
        • Asar a la parrilla y seguridad alimentaria
        • Cleanliness Helps Prevent Foodborne Illness
        • Cocción Versus Seguridad
        • Cocinar en Hornos Microondas
        • Cooking for Groups
        • Cooking with Microwave Ovens
        • Cutting Boards
        • Cómo Encontrar el Número de Establecimiento del USDA (EST) en el Empaque de Alimentos
        • Cómo las Temperaturas Afectan a los Alimentos
        • Danger Zone 40F - 140F
        • Deep Fat Frying
        • Doneness Versus Safety
        • El Color de la Carne y Las Aves
        • El Gran Deshielo: Métodos Seguros para Descongelar
        • El Manejo Adecuado de los Alimentos Pedidos Por Correo
        • Entendiendo las Retiradas de Alimentos del Mercado del FSIS
        • Fechas en Productos Alimenticios
        • Food Allergies: The “Big 9”
        • Food Thermometers
        • Freidoras de Aire y Seguridad Alimentaria
        • Freír en Grasa y Seguridad Alimentaria
        • Glosario de Términos de Empaque
        • Glossary of Packaging Terms
        • Grilling Food Safely
        • Grilling and Food Safety
        • Guía del Consumidor Sobre Inocuidad Alimentaria: Tormentas y Huracanes Severos
        • High Altitude Cooking
        • Hongos en los Alimentos: ¿Son Peligrosos?
        • How Temperatures Affect Food
        • How to Find the USDA Establishment Number
        • Importación de Productos Cárnicos, Avícolas y Ovoproductos a Estados Unidos
        • Importing Meat, Poultry & Egg Products US
        • Irradiation and Food Safety FAQ
        • Keeping "Bag" Lunches Safe
        • Keeping Food Safe During an Emergency
        • La Cocción En Elevaciones Altas
        • La Congelación Y Seguridad Alimentaria
        • La Limpieza Ayuda a Prevenir Enfermedades Transmitidas Por Los Alimentos
        • Las Ollas de Cocción Lenta y la Seguridad Alimentaria
        • Las Sobras de Comida e Inocuidad Alimentaria
        • Lavado de Alimentos: ¿Promueve la Inocuidad Alimentaria?
        • Mail Order Food Safety
        • Manipulación Adecuada de Alimentos Para Llevar
        • Mantenga los Alimentos Seguros - Conceptos Básicos de Inocuidad Alimentaria
        • Manteniendo Seguros Los Almuerzos En "Bolsas"
        • Materiales de Empaque para Carnes y Aves
        • Meat and Poultry Labeling Terms
        • Meat and Poultry Packaging Materials
        • Molds on Food: Are They Dangerous?
        • Natural Flavors on Meat and Poultry Labels
        • Preguntas Frecuentes Sobre Irradiación y Seguridad Alimentaria
        • Preguntas y Respuestas Sobre la Seguridad Alimentaria Durante un Tailgate
        • Sabores Naturales de Carnes y Aves en las Etiquetas
        • Safe Handling of Take-Out Foods
        • Seguridad Alimentaria Durante Caminatas, Campamentos y Paseos en Bote
        • Seguridad Alimentaria de Comida No Perecedera
        • Slow Cookers and Food Safety
        • Smoking Meat and Poultry
        • Tabla de Temperatura Interna Mínima Segura
        • Tablas de Cortar
        • Tailgating Food Safety Q & A
        • Termómetros para Alimentos
        • Termómetros para Electrodomésticos
        • Términos de Etiquetado de Carnes y Aves
        • Understanding FSIS Food Recalls
        • Voluntarios Sobre la Seguridad Alimentaria
        • Water in Meat & Poultry
        • Zona de Peligro (40 F - 140 F)
        • Food Product Dating
        • Freezing and Food Safety
        • Leftovers and Food Safety
        • Refrigeration
          • La Refrigeración y Seguridad Alimentaria
        • Safe Temperature Chart
        • Shelf-Stable Food
        • The Big Thaw — Safe Defrosting Methods
        • The Color of Meat and Poultry
        • Washing Food: Does it Promote Food Safety?
        • Food Safety While Hiking, Camping & Boating
      • Meat & Catfish
        • Bacon and Food Safety
        • Bagre de la Granja a la Mesa
        • Beef From Farm To Table
        • Bison from Farm to Table
        • Bisonte de la Granja a la Mesa
        • Cabra de la Granja a la Mesa
        • Carne Seca y Seguridad Alimentaria
        • Carne de Cerdo Fresca de la Granja a la Mesa
        • Carne de Res de la Granja a la Mesa
        • Carne de Ternera de la Granja a la Mesa
        • Carne de res ablandada mecánicamente
        • Carne en Conserva y Seguridad Alimentaria
        • Catfish from Farm to Table
        • Color de la Carne Molida Cocida en Relación Con El Grado De Cocción
        • Color of Cooked Ground Beef as It Relates to Doneness
        • Conejo de la Granja a la Mesa
        • Cordero de la Granja a la Mesa
        • Corned Beef
        • Cuando se Asan “Otras” Carnes para las Fiestas
        • Door-to-Door Meat Sales
        • El Jamón y la Seguridad Alimentaria
        • El Tocino y la Seguridad Alimentaria
        • Fresh Pork from Farm to Table
        • Goat from Farm to Table
        • Ground Beef and Food Safety
        • Hams and Food Safety
        • Hot Dogs & Food Safety
        • Jerky
        • La Carne Molida y la Seguridad Alimentaria
        • Lamb From Farm to Table
        • Las Salchichas “hot dogs” y la Seguridad Alimentaria
        • Los Embutidos y Seguridad Alimentaria
        • Mechanically Tenderized Beef
        • Rabbit From Farm to Table
        • Roasting Those "Other" Holiday Meats
        • Sausages and Food Safety
        • Veal from Farm to Table
        • Venta de Carne a Domicilio
        • Yersiniosis and Chitterlings Tips
        • Yersiniosis y los Chinchulines (Tripas): Consejos Para Protegerlo de Enfermedades Transmitidas Por Alimentos
      • Poultry
        • Happy Thanksgiving!
        • Amarras para las Aves y Otros Accesorios
        • Aves: Baños en Solución, Salmueras y Marinadas
        • Chicken From Farm to Table
        • Chicken Liver
        • Duck and Goose from Farm to Table
        • El Relleno y Seguridad Alimentaria
        • Hablemos Sobre el Pavo: Una Guía Para el Consumidor Sobre Cómo Asar un Pavo de Forma Segura
        • Hock Locks and Other Accoutrements
        • Is Pink Turkey Meat Safe?
        • La Etiqueta del Ave Dice “Fresco”
        • La Preparación de Turduckens Requiere un Manejo Adecuado
        • Let's Talk Turkey Roasting
        • Pato y Ganso de la Granja a la Mesa
        • Pavo de la Granja a la Mesa
        • Pavo: Rutas Alternativas Hacia la Mesa
        • Pollo de la Granja a la Mesa
        • Poultry Processing: Questions & Answers
        • Poultry: Basting, Brining, and Marinating
        • Preparación Básica del Pavo: Cocinar De Forma Adecuada
        • Preparación Básica del Pavo: Como Descongelar Seguramente
        • Preparación Básica del Pavo: El Relleno
        • Preparación Básica del Pavo: Manejo de las Comidas Cocidas
        • Procesamiento de Aves: Preguntas y Respuestas
        • Stuffing and Food Safety
        • The Poultry Label Says "Fresh"
        • Turduckens Require Safe Food Handling
        • Turkey Basics: Handling Cooked Dinners
        • Turkey Basics: Safe Cooking
        • Turkey Basics: Safe Thawing
        • Turkey Basics: Stuffing
        • Turkey from Farm to Table
        • Turkey: Alternate Routes to the Table
        • ¿Es Segura la Carne de Pavo Rosada?
      • Eggs
        • Egg Products and Food Safety
        • Huevos en Cascarón De la Granja a la Mesa
        • Ovoproductos e Inocuidad Alimentaria
        • Shell Eggs from Farm to Table
      • Emergencies
        • A Consumer's Guide to Food Safety: Severe Storms and Hurricanes
        • Eliminando Olores de Refrigeradores y Congeladores
        • Fires and Food Safety
        • Incendios y Seguridad Alimentaria
        • Keep Your Food Safe During Emergencies
        • Removing Odors from Refrigerators and Freezers
      • USDA Meat and Poultry Hotline
      • Brochures & Publications
    • Food Defense and Emergency Response
      • Emergency Response
      • Continuity of Operations (COOP)
      • Food Defense
        • Risk Mitigation Tool
        • Food Defense Considerations for Transportation of FSIS-Regulated Products
        • Food Defense Tools, Resources and Training
        • Functional Food Defense Plans
        • International Food Defense
  • Science & Data
    • Research Priorities
      • FSIS/ORISE Food Safety Fellowship Program
        • Aaron Dudley
        • Lauren Lee
        • Sharon Nieves-Miranda
        • Yesutor Soku
        • Ali Strickland
    • Data Sets & Visualizations
      • Microbiology
        • Baseline Microbiology Data Reports
        • Microbiological Testing Program for RTE Meat and Poultry Products
          • Tables & Results Microbiological Testing Program for RTE Meat
          • Tables & Results: Microbiological Testing Program Pasteurized Egg Products
          • Aggregate Salmonella Categorization of Raw Chicken Parts, NRTE Comminuted Poultry, Young Chicken Carcass and Young Turkey Carcass Establishments Using Moving Windows
          • Salmonella Initiative Program Criteria
            • Quarterly Sampling Reports on Antimicrobial Resistance
            • Quarterly Sampling Reports on Raw Beef Products
            • Quarterly Sampling Reports on Ready-to-eat Products and Egg Products
            • Quarterly Sampling Reports on Salmonella
            • Salmonella Action Plan: A One and Two Year Update
        • Microbiological Testing Program for Escherichia coli O157:H7 and non-O157 Shiga toxin-producing Escherichia coli (STEC)
          • Year-to-Date Totals: Testing of Raw Ground Beef Component (RGBC) Samples for E. coli O157:H7 and non-O157 Shiga toxin-producing E. coli (STEC)
          • Annual Report for STEC in Raw Ground Beef or Veal and Raw Ground Beef or Veal Components
          • Individual E. coli Positive Results for Raw Ground Beef (RGB) and RGB Components 2017
          • Individual E. coli Positive Results for Raw Ground Beef (RGB) and RGB Components 2018
          • Individual E. coli Positive Results for Raw Ground Beef (RGB) and RGB Components 2016
          • Individual E. coli Positive Results for Raw Ground Beef (RGB) and RGB Components 2015
          • Year-to-Date 2018 Totals: Results of Raw Ground Beef Component (RGBC) Samples for E. coli O157:H7 and non-O157 Shiga toxin-producing E. coli (STEC):
        • Salmonella Verification Testing Program Monthly Posting
      • Chemical Residues and Contaminants
      • Humane Handling Data
      • Laboratory Sampling Data
        • Egg Product Testing, Years 1995-2017
      • Inspection Task Data
    • Developer Resources
      • Recall API
    • Scientific Reports
      • Public Health Regulations (PHR)
        • FSIS Data Analysis and Reporting: Public Health Regulations FY 2025
        • FSIS Data Analysis and Reporting: Public Health Regulations FY 2024
        • FSIS Data Analysis and Reporting: Public Health Regulations FY 2023
        • FSIS Data Analysis and Reporting: Public Health Regulations FY 2022
        • FSIS Data Analysis and Reporting: Public Health Regulations FY 2021
        • FSIS Data Analysis and Reporting: Public Health Regulations FY 2020
        • FSIS Data Analysis and Reporting: Public Health Regulations FY 2019
        • FSIS Data Analysis and Reporting: Public Health Regulations FY 2018
        • FSIS Data Analysis and Reporting: Public Health Regulations FY 2017
        • FSIS Data Analysis and Reporting: Public Health Regulations FY 2016
      • Interagency Food Safety Analytics Collaboration (IFSAC)
    • Laboratories & Procedures
      • Accredited Laboratory Program
        • Key Facts: ISO Accreditation
      • FSIS Laboratories
        • Requesting Bacterial Isolates from FSIS
    • Risk Assessments
    • Sampling Program
      • Raw Pork Products Exploratory Sampling Program
      • Sampling Results for FSIS Regulated Products
    • National Antimicrobial Resistance Monitoring System (NARMS)
    • Journal Publications
  • Policy
    • Food Safety Acts
      • Federal Meat Inspection Act
      • Poultry Products Inspection Act
      • Egg Products Inspection Act
      • Humane Methods of Slaughter Act
    • FSIS Guidelines
    • Directives & Notices
      • FSIS Notices
      • FSIS Directives
    • Petitions
    • Federal Register & Rulemaking
      • Federal Register Notices
      • Federal Register Rules
      • Executive Orders, Small Business Protection Laws & Other Guidance
      • Regulatory Priorities
    • Advisory Committees
      • National Advisory Committee on Meat and Poultry Inspection (NACMPI)
      • National Advisory Committee on Microbiological Criteria For Foods (NACMCF)
        • 2023-2025 National Advisory Committee on Microbiological Criteria for Foods (NACMCF)
        • 2021-2023 National Advisory Committee on Microbiological Criteria For Foods (NACMCF)
        • NACMCF 2022 Subcommittee
        • 2018-2020 National Advisory Committee on Microbiological Criteria For Foods (NACMCF)
    • FSIS Procurement
    • National Federal Financial Assistance
    • Regulatory Decisions & Non-Retaliation
  • Inspection
    • Inspection Programs
      • Inspection of Meat Products
        • Humane Handling Ombudsman
        • Modernization of Swine Slaughter Inspection
      • Inspection of Poultry Products
        • Reducing Salmonella in Poultry
          • Pilot Projects: Salmonella Control Strategies
          • Proposed Regulatory Framework to Reduce Salmonella Illnesses Attributable to Poultry
            • Component 1
            • Component 2
            • Component 3
          • Salmonella By the Numbers
          • Salmonella KPI
          • Salmonella Risk Assessments
        • Modernization of Poultry Slaughter Inspection
      • Inspection of Egg Products
      • Inspection of Siluriformes
    • Compliance Guidance
      • Significant Guidance
      • HACCP
        • HACCP-Based-Inspection Models Project
          • New Poultry Inspection System (NPIS)
          • HIMP Redesign Achievement of Performance Standards Young Chicken Plants
          • List of HIMP Participating Plants
        • HACCP Validation
      • PHIS
        • PHIS: Historical Information
      • Retail Guidance
      • Small & Very Small Plant Guidance
        • Appealing Inspection Decisions
        • Food Safety Resources for Small and Very Small Plant Outreach: Order Form
        • Small Plant Help Desk
        • Small Plant Help Desk Form
      • Microbial Risk
        • Listeria Monocytogenes
        • Salmonella
        • Shiga Toxin-Producing E.Coli (STEC) and E. Coli O157:H7
        • Specified Risk Material
          • BSE Rules Being Strictly Enforced
            • BSE Workshops for Small & Very Small Plants
          • FSIS BSE Resources
      • Specified Risk Material Resources
      • Food Safety Assessments Tools
      • Recall Process
      • Sanitation Performance Standards Compliance Guide
      • Labeling
        • Basics of Labeling
        • Claims Guidance
        • Nonfood Compounds
          • Compounds Used for Construction and Repair in Federally Inspected Meat and Poultry Plants
          • Criteria Used by the Former Compounds and Packaging Branch for Evaluating Nonfood Compounds and Proprietary Substances
        • Ingredients Guidance
        • Label Submission and Approval System (LSAS)
          • Integration of Paper Label Applications into the Label Submission and Approval System (LSAS)
        • Labeling Policies
          • Human Food Made with Cultured Animal Cells
          • Regulations for Package Dating
          • Comprehensive List of Reasons for Label Modifications and Returns
        • Labeling Procedures
          • Information Required For Requesting a Temporary Approval
          • 10 Most Common Mistakes And How to Avoid Them
          • Label Submission Checklist
          • Labeling Situations That Can Not Have a Temporary Approval
          • Labeling and Establishment Responsibilities
          • Ten Most Commonly Asked Labeling Questions
          • Trans Fat Declarations in the Nutrition Facts Panel on Product Labeling
      • New Technology
        • Cooperative Agreements FY 2003
        • Cooperative Agreements FY 2004
        • Cooperative Agreements FY 2005
        • Food Safety Technologies FY 2003
        • Food Safety Technologies FY 2004
        • Food Safety Technologies FY 2005
        • NOL for Non-O157 STEC Test Methods
        • New Technology Information Table
      • Humane Handling
    • Import & Export
      • Import & Export Library
        • Eligible Foreign Establishments
        • Eligible U.S. Establishments by Country
          • Australia Eligible Establishments
          • Hong Kong Lamb Export Eligible Establishments
          • Hong Kong Pork Export Eligible Establishments
          • Hong Kong Poultry Export Eligible Establishments
          • Brazil Export Eligible Establishments
          • Egypt Export Eligible Establishments
          • Hong Kong Beef Export Eligible Establishments
          • Israel Export Eligible Establishments
          • Japan (Casings) Export Eligible Establishments
          • Japan (Cold Storage Facilities) Export Eligible Establishments
          • Mexico Export Eligible Establishments
          • Russia (Beef) Export Eligible Establishments
          • Russia (Pork) Export Eligible Establishments
          • Russia (Poultry) Export Eligible Establishments
          • Russia (Prepared Products) Export Eligible Establishments
          • South Africa Eligible Establishments
      • Import Guidance
        • FSIS Import Procedures for Meat, Poultry & Egg Products
        • FSIS Import Reinspection
        • Sourcing Egg Products and Shell Eggs From Foreign Countries
      • Export Guidance
      • Equivalence
      • PHIS Components
      • International Reports
        • Foreign Audit Reports
        • Import and Export Data
    • Regulatory Enforcement
      • Humane Handling Enforcement
      • Quarterly Enforcement Reports
    • Inspection Training & Videos
      • Humane Interactive Knowledge Exchange (HIKE) Scenarios
      • Inspection & Mission Training
      • Meat, Poultry and Egg Product Inspection Videos
      • Regulatory Education Video Seminars
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      • Grants & Financial Options
    • State Inspection Programs
      • Cooperative Interstate Shipping Program
        • Cooperative Interstate Shipment (CIS) Establishments
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      • States With and Without Inspection Programs
      • Reviews of State Programs
    • Establishments
      • FSIS Inspected Establishments
      • Meat, Poultry and Egg Product Inspection Directory
    • Inspection Forms
  • About FSIS
    • History
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Guidebooks
03/14/2025, 08:00AM EDT

Chemistry Laboratory Guidebook

The Chemistry Laboratory Guidebook (CLG) contains test methods used by FSIS Field Service Laboratories to support FSIS regulatory activities. The CLG contains methods for the analysis of food composition, food additives, nutrients, veterinary drug and pesticide residues. Methods contained in the CLG are designed to provide FSIS analysts with written documentation to facilitate training, performance, quality assessment, and interpretation of data.  FSIS does not specifically endorse any of the products mentioned in the CLG and acknowledges other products may be available for laboratory use.

Definitions for Chemistry Methods

Terms used in CLG methods are defined here


Allergen Verification | Food Chemistry | Inorganic Chemistry | Laboratory Introduction | Residue Chemistry (Inactive) | Residue Chemistry (Infrequent) | Residue Chemistry (Routine) | Siluriformes Species Identification


Laboratory Introduction

Method
Number
Method Title Effective
Date
CLG-1 FSIS Laboratory System Introduction, Method Performance Expectations, and Sample Handling for Chemistry 2023-01-16

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Residue Chemistry (Routine)

Method
Number
Method Title Effective
Date
CLG-MEGA1

Screening of Veterinary Drug and Pesticides Residues by UHPLC-MS/MS

  • CLG-MEGA1 Appendix 1.00 Flow Chart Specific for FSIS Laboratory Screening of Animal Drug Residues and Pesticides by UPLC-MS-MS (Effective Sep 30, 2024; PDF Only)
2024-12-23
CLG-MRQ1

Quantitation and Confirmation of Animal Drug Residues by High-Resolution Accurate-Mass Liquid Chromatography Mass Spectrometry (HRAM LC-MS)

  • CLG-MRQ1 Appendix 1.01 Flow Chart Specific for FSIS Laboratory Quantitation for Animal Drug Residues by HRAM LC-MS (Effective Nov 1, 2024; PDF Only)
2024-11-01
CLG-MRM3 Screening and Confirmation of Animal Drug Residues by UHPLC-MS-MS (Effective Jul 15, 2024; PDF Only)
  • CLG-MRM3 Appendix 1.00 Flow Chart Specific for FSIS Laboratory Screening and Confirmation of Animal Drug Residues by UHPLC-MS-MS (Effective Feb 28, 2023; PDF Only)
2024-07-15
CLG-PFAS2

Screening, Determination and Confirmation of PFAS by UPLC-MS-MS

  • CLG-PFAS2 Appendix 1.00 Flow Chart Specific for FSIS Laboratory Screening, Determination, and Confirmation of PFAS by UHPLC-MS-MS (Effective Feb 28, 2023; PDF Only)
2023-02-28
CLG-AMG2 Screening and Confirmation for Aminoglycosides by LC-MS-MS 2021-02-15
CLG-AMG4

Screening for Aminoglycosides by UHPLC-MS/MS

  • CLG-AMG4 Appendix 1.00 Flow Chart Specific for FSIS Laboratory Screening for Aminoglycosides by UHPLC-MS-MS (Effective Feb 28, 2023; PDF Only)
2024-12-23
CLG-PST5 Screening for Pesticides by UHPLC-MS/MS and GC-MS/MS (Effective Jun 17, 2024; PDF Only)
  • CLG-PST5 Appendix 1.00 Flow Chart Specific for FSIS Laboratory Screening for Pesticides by UHPLC-MS-MS and GC-MS-MS
2024-06-17
CLG-ADD3 Inhibition Screen Test for Antimicrobial Drugs 2021-04-26
CLG-AGON1

Screening Determination and Confirmation of β-Agonists by HPLC-MS-MS

  • CLG-AGON1 Appendix 1.00 Flow Chart Specific for FSIS Laboratory Screening Quantitation and Confirmation of Beta-Agonists by UHPLC-MS-MS (Effective Sep 11, 2023; PDF Only)
2023-09-11
CLG-AVR Quantitation of Doramectin, Ivermectin, and Moxidectin by UHPLC
  • CLG-AVR Appendix 1.00 Flow Chart Specific for FSIS Laboratory Quantitation of Doramectin, Ivermectin, and Moxidectin by UHPLC (Effective May 27, 2024; PDF Only)
2024-05-27
CLG-AVR2 Confirmation of Avermectins by UHPLC-MS/MS
  • CLG-AVR2 Appendix 1.00 Flow Chart Specific for FSIS Laboratory Confirmation of Avermectins by UHPLC-MS/MS (Effective May 27, 2024; PDF Only)
2024-05-27
CLG-AVR1 Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometric (LC/APCI/MS) Confirmation of Ivermectin, Doramectin and Moxidectin 2011-03-31
CLG-CAM Determination and Confirmation of Chloramphenicol 2018-04-13
CLG-CBX4 Screen of Carbadox Metabolite QCA by HPLC-MS-MS 2016-03-31
CLG-FLOR1 Determination and Confirmation of Florfenicol 2021-03-09
CLG-HRM Screening and Determination of Multiple Hormones in Beef Muscle by LC/MS/MS 2018-11-05
CLG-MGCV3 Determination and Confirmation of Malachite Green and Crystal violet by UHPLC-MS-MS 2019-05-12
CLG-NFUR3 Screening and Confirmation of Four Nitrofuran Metabolites by Liquid Chromatography-Tandem Mass Spectrometry 2016-03-07
CLG-TOX1 Identification of Poisons and Toxins by GC-MS 2013-05-05
CLG-TOX2

Screening of Toxins by LC-MS/MS

  • CLG-TOX2 Appendix 1.00 Flow Chart Specific for FSIS Laboratory Screening of Toxins by LC-MS-MS (Effective Feb 28, 2023; PDF Only)
2023-02-28

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Allergen Verification

Method
Number
Method Title Effective
Date
CLG-ALG1

Screening and Confirmation of Allergens by XMAP® Food Allergen Detection Assay

  • CLG-ALG Appendix 1.00 Flow Chart Specific for FSIS Laboratory Screening and Confirmation of Allergens by XMAP
2025-02-12

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Food Chemistry

Method
Number
Method Title Effective
Date
CLG-MOI

Quantitation of Moisture

  • CLG-MOI Appendix 1.00 Flow Chart Specific for FSIS Laboratory Quantitation of Moisture (Effective Dec 28, 2023; PDF Only)
2023-12-28
CLG-PRO4

Quantitation of Protein by Combustion

  • CLG-PRO4 Appendix 1.00 Flow Chart Specific for FSIS Laboratory Quantitation of Protein by Combustion (Effective Dec 28, 2023; PDF Only)
2023-12-28
CLG-FAT

Quantitation of Fat

  • CLG-FAT Appendix 1.00 Flow Chart Specific for FSIS Laboratory Quantitation of Fat (Effective Aug 28, 2023; PDF Only)
2023-08-28
CLG-SLT

Quantitation of Salt

  • CLG-SLT Appendix 1.00 Flow Chart Specific for FSIS Laboratory Quantitation of Salt (Effective Aug 28, 2023; PDF Only)
2023-08-28

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Inorganic Chemistry

Method
Number
Method Title Effective
Date
CLG-TM3 Determination of Metals by ICP-MS and ICP-OES (Optical Emission Spectrometry) 2018-11-05
CLG-TM4 Detection of Thallium, Arsenic and Mercury using ICP-AES 2006-12-11
CLG-CAL Calcium/Tritimetric 1993-05
CLG-CAL2 Calcium, Inductively Coupled Plasma (ICP) Determination 2003-01-10
CLG-MER Determination of Mercury by Atomic Absorption Spectrophotometry 1991-07
CLG-MERC1 Determination of Mercury by Atomic Absorption Spectroscopy 2018-04-13
CLG-SOCAL3 Determination of Sodium and Calcium by ICP Atomic Emission Spectroscopy 2014-09-25

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Siluriformes Species Identification

Method
Number
Method Title Effective
Date
CLG-FPCR Fish Speciation by PCR/Sequencing of Mitochondrial Cytochrome Oxidase I (COI) Gene 2020-04-30

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Infrequent Chemistry Methods

Method
Number
Method Title Effective
Date
CLG-CEF2 Quantitation and Confirmation of Desfuroylceftiofur Cysteine Disulfide by LC-MS-MS 2020-12-07
CLG-FLQ2 Confirmation of Fluoroquinolone Antibiotics by HPLC Ion Trap Mass Spectrometry 2021-04-26
CLG-FLX4 Determination and Confirmation of Flunixin by HPLC/ESI-MS/MS 2021-03-15
CLG-ICT1 Internal Cooking Temperature 1993-05
CLG-ICT2 Internal Cooking Temperature/Coagulation 1993-05
CLG-PENG1 Determination and Confirmation of Penicillin G by LC-MS/MS 2020-12-07
CLG-PHS1 Determination of Phosphate 2009-08-10
CLG-SFT1 Determination of Sulfites 2006-09-05
CLG-SUL4 Screening, Quantitation and Confirmation of Sulfonamides by Liquid Chromatography - Tandem Mass Spectrometry (LC-MS-MS) 2021-02-01
CLG-TIL1 Determination of Tilmicosin by HPLC/Ion Pairing 2021-03-15

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Inactive Chemistry Methods

Method
Number
Method Title Effective
Date
CLG-AMG1 Confirmation of Aminoglycosides by HPLC-MS/MS 2011-06-03
CLG-ANA Determination and Confirmation of Diethylstilbestrol (DES) and Zeranol by GC/MS 2004-11-10
CLG-ARS Determination of Arsenic by Atomic Absorption Spectroscopy 2016-06-20
CLG-BLAC Screening and Confirmation of β-Lactam Antibiotics by HPLC-MS/MS 2011-07-02
CLG-BSP Determination of Benzoic Acid, Sorbic Acid, and Methyl, Ethyl, Propyl, and Butyl Parabens by HPLC 2004-11-01
CLG-CAM1 Screening for Chloramphenicol by ELISA 2018-04-13
CLG-CEF1 Determination for Ceftiofur-Related (DCA) Residues by HPLC 2012-10-01
CLG-FLOR2 Confirmation of Florfenicol by GC/MS 2006-08-07
CLG-FLX3 Screening of Flunixin Residues by ELISA 2011-03-10
CLG-MAL1 Confirmation of Macrolide/Lincosamide Antibiotics by Ion Trap HPLC/MS/MS 2007-07-30
CLG-MGCV1 Screening for Malachite Green and Crystal Violet by ELISA 2016-06-20
CLG-MGCV2 Confirmation of Malachite Green and Crystal Violet by UHPLC-MS-MS 2019-02-07
CLG-MRM1 Screening and Confirmation of Animal Drug Residues by UHPLC-MS-MS ("Multi-Residue Method") 2018-11-05
CLG-MRM2 Screening and Confirmation of Animal Drug Residues by UHPLC-HRMS-MS 2017-08-21
CLG-NFUR2 Screening and Confirmation of Nitrofuran Metabolites by Liquid Chromatography-Tandem Mass Spectrometry 2010-03-09
CLG-NIMZ1 Screen for Nitroimidazoles by HPLC 2005-12-20
CLG-NIMZ2 Confirmation of Nitroimidazoles by ESI-LC/MS/MS 2005-02-07
CLG-PBZ2 Confirmation of Phenylbutazone by LC/ESI-MS/MS 2007-12-10
CLG-PBZ3 Screening of Phenylbutazone by ELISA 2013-05-15
CLG-TET2 Qualitative Identification of Tetracyclines 2011-09-19
CLG-TIL2 Confirmation of Tilmicosin by APCI-LC/MS3 2002-03-15
CLG-TRN Determination of Trenbolone by ELISA 2004-11-30
CLG-TRN1 Confirmation of α and β-Trenbolone by GC/MS/MS 2005-01-03
CLG-ZRL Screening for Zeranol by ELISA 2007-02-26
CLG-CBX1 Determination of Carbadox Metabolite by GC/ECD 2010-03-24
CLG-CBX2 Confirmation of Carbadox Metabolite in Swine Liver by GC/EI Ion-trap MS 2005-08-22
CLG-CHC3 Determination of Chlorinated Hydrocarbons (CHCs) and Chlorinated Organophosphate Hydrocarbons (COPs) with Gel Permeation Chromatography (GPC) 2010-06-01
CLG-CLN3 ELISA Screening for β-Agonist Residues in Animal Retinal Tissue 2004-07-19
CLG-CYN Cyanide in Tissue, Processed Meats, and Serum 1991-07
CLG-DEX Dexamethasone 1991-07
CLG-DPN1 Determination of Dipyrone-Related Residues by HPLC 2004-10-25
CLG-EDB Ethylene Dibromide 1991-07
CLG-FET Fenthion 1991-07
CLG-FLX1 Determination of Flunixin in Cattle Liver by HPLC 2002-11-04
CLG-FLX2 Confirmation of Flunixin Residues in Bovine Liver by HPLC/ESI-MS/MS 2003-03-24
CLG-GAS Gasoline 1991-07
CLG-GEN Gentamicin in Porcine Tissue by HPLC 1991-07
CLG-HEC1 Screen for Halogenated Environmental Contaminants 2005-10-14
CLG-HLF Halofuginone 1991-07
CLG-LAS Lasalocid in Bovine Tissue 1991-07
CLG-LSD Lysergic Acid Diethylamine and Phencyclidine 1991-07
CLG-LVD Cyromazine and Melamine 1991-07
CLG-MEL1 Determination and Confirmation of Melamine by LC/MS/MS 2008-12-02
CLG-MGA1 Confirmation of Melengestrol Acetate by APCI/LC/MS3 2003-03-18
CLG-MGA2 Screen for Melengestrol Acetate in Fat Using ELISA 2006-02-01
CLG-MGA4 Determination of Melengestrol Acetate 2007-12-12
CLG-MON Monensin 1991-07
CLG-MPT Morantel and Pyrantel Drug-Related Metabolites 1991-07
CLG-NAR Narasin in Cattle and Chicken Tissues 1991-07
CLG-NBV Novobiocin and Virginiamycin 1991-07
CLG-NFUR1 Screen for Furazolidone and Furaltadone Metabolites by ELISA 2005-03-07
CLG-NIC Nicarbazin in Poultry Tissues 1991-07
CLG-NTR2 Nitrosamines/Low-Temperature Vacuum Distillation Procedure 1991-07
CLG-NTR3 Analysis of Nitrosamines using Supercritical Fluid Extraction 2009-06-30
CLG-PFC1 Determination and Confirmation of PFOA and PFOS by UPLC/MS/MS 2009-11-05
CLG-PST4 Confirmation of Pesticides by GC/MS/MS 2010-08-30
CLG-PYRE Synthetic Pyrethrins/c-ELISA 1991-07
CLG-RAC1 Determination of Ractopamine Hydrochloride by High Performance Liquid Chromatography 2013-05-03
CLG-SUL Determination and Confirmation of Sulfonamides 2009-09-25
CLG-SUL2 Automated Robotic Extraction/TLC Analysis for Sulfonamide Residues in Animal Tissues, Eggs, and Egg Products 2011-05-11
CLG-SUL3 Screening of Sulfonamides by Automated Robotic Extraction and Thin Layer Chromatography (TLC) 2012-02-24
CLG-TST2 Screening and Confirmation of Thyreostats by HPLC/MS/MS 2009-01-22

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All methods are offered as PDF documents. If you require an alternative format, please contact:

Chemistry Section
Laboratory Quality Assurance Response and Coordination Staff (LQARCS), USDA/FSIS/OPHS
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Athens, GA 30605 
Phone: (706) 713-5898 

Last Updated: Mar 14, 2025
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