The Microbiology Laboratory Guidebook (MLG) contains test methods used by the FSIS Field Service Laboratories to support FSIS regulatory activities.  The MLG contains methods for the sample preparation, isolation and identification of the major foodborne pathogenic microorganisms and their toxins, meat tissue species identification, and the detection of antimicrobial residues. Appendices to the MLG include a pathogen method summary chart, method flow charts, Media and Reagent formulations, and Most Probable Number Tables. Methods contained in the MLG are designed to provide FSIS analysts with written documentation to facilitate training, performance, quality assessment, and interpretation of data. FSIS does not specifically endorse any of the products mentioned in the MLG and acknowledges other products may be available for laboratory use. 

All methods are offered as PDF documents. If you require an alternative format, please contact: Microbiology Section Laboratory Quality Assurance Staff, USDA/FSIS/OPHS 950 College Station Road Athens, GA 30605 Phone: (706) 713-5898