FSIS conducted an analysis to evaluate whether semicarbazide (SEM) could be detected in fresh and frozen chicken samples collected at various points during production. Tissue-bound SEM has been used as a target for monitoring compliance with the restrictions on nitrofurazone use. Nitrofurazone is a nitrofuran antibiotic that is restricted from use in food-producing animals. Its metabolism results in tissue-bound metabolites, including semicarbazide (SEM).