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Chemistry Laboratory Guidebook

The Chemistry Laboratory Guidebook (CLG) contains test methods used by FSIS laboratories to support the Agency's inspection program, ensuring the safety of meat, poultry, and processed egg products. The CLG contains methods for the analysis of food composition, food additives, nutrients, veterinary drug and pesticide residues. Methods are designed to provide analysts with written documentation to facilitate training, performance, quality assessment, and interpretation of data. 

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Contents

Methods listed in this index are grouped as follows:

  Residue Chemistry (Routine)

Method
Number
Method Title Effective
Date
CLG-MRM1 Screening and Confirmation of Animal Drug Residues by UHPLC-MS-MS ("Multi-Residue Method") 2016-03-07
CLG-AMG2 Screening and Confirmation for Aminoglycosides by LC-MS-MS 2015-04-06
CLG-AMG4 Screening for Aminoglycosides by LC-MS-MS 2015-11-27
CLG-PST5 Screening for Pesticides by LC/MS/MS and GC/MS/MS 2016-03-14
CLG-ADD3 Inhibition Screen Test for Antimicrobial Drugs 2012-08-19
CLG-AGON1 Screening, Determination, and Confirmation of β-Agonists by HPLC-MS-MS 2016-01-20
CLG-AVR Determination of Ivermectin, Doramectin, and Moxidectin by HPLC 2011-03-28
CLG-AVR1 Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometric (LC/APCI/MS) Confirmation of Ivermectin, Doramectin and Moxidectin 2011-03-31
CLG-CAM Determination and Confirmation of Chloramphenicol 2013-09-09
CLG-CBX4 Screen of Carbadox Metabolite QCA by HPLC-MS-MS 2016-03-31
CLG-CEF2 Quantitation and Confirmation of Desfuroylceftiofur Cysteine Disulfide by LC-MS-MS 2016-07-24
CLG-FLOR1 Determination and Confirmation of Florfenicol 2010-10-12
CLG-FLOR2 Confirmation of Florfenicol by GC/MS 2006-08-07
CLG-FLX4 Determination and Confirmation of Flunixin by HPLC/ESI-MS/MS 2012-07-16
CLG-HRM Determination of Multiple Hormones in Beef Muscle by LC/MS/MS 2014-09-15
CLG-MGCV1 Screening for Malachite Green and Crystal Violet by ELISA 2016-06-20
CLG-MGCV2 Confirmation of Malachite Green and Crystal Violet by UHPLC-MS-MS 2016-06-20
CLG-NFUR3 Screening and Confirmation of Four Nitrofuran Metabolites by Liquid Chromatography-Tandem Mass Spectrometry 2016-03-07
CLG-PENG1 Determination and Confirmation of Penicillin G by LC-MS/MS 2016-09-19
CLG-SUL4 Screening, Quantitation and Confirmation of Sulfonamides by Liquid Chromatography - Tandem Mass Spectrometry (LC-MS-MS) 2016-03-14
CLG-TIL1 Determination of Tilmicosin by HPLC/Ion Pairing 2015-03-30
CLG-TOX1 Identification of Poisons and Toxins by GC-MS 2013-05-05

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Residue Chemistry (Infrequent)

Method
Number
Method Title Effective
Date
CLG-AMG1 Confirmation of Aminoglycosides by HPLC-MS/MS 2011-06-03
CLG-ANA Determination and Confirmation of Diethylstilbestrol (DES) and Zeranol by GC/MS 2004-11-10
CLG-BLAC Screening and Confirmation of β-Lactam Antibiotics by HPLC-MS/MS 2011-07-02
CLG-BSP Determination of Benzoic Acid, Sorbic Acid, and Methyl, Ethyl, Propyl, and Butyl Parabens by HPLC 2004-11-01
CLG-CAM1 Screening for Chloramphenicol by ELISA 2013-04-15
CLG-CEF1 Determination for Ceftiofur-Related (DCA) Residues by HPLC 2012-10-01
CLG-FLQ2 Confirmation of Fluoroquinolone Antibiotics by HPLC Ion Trap Mass Spectrometry 2006-01-13
CLG-FLX3 Screening of Flunixin Residues by ELISA 2011-03-10
CLG-MAL1 Confirmation of Macrolide/Lincosamide Antibiotics by Ion Trap HPLC/MS/MS 2007-07-30
CLG-NFUR2 Screening and Confirmation of Nitrofuran Metabolites by Liquid Chromatography-Tandem Mass Spectrometry 2010-03-09
CLG-NIMZ1 Screen for Nitroimidazoles by HPLC 2005-12-20
CLG-NIMZ2 Confirmation of Nitroimidazoles by ESI-LC/MS/MS 2005-02-07
CLG-PBZ2 Confirmation of Phenylbutazone by LC/ESI-MS/MS 2007-12-10
CLG-PBZ3 Screening of Phenylbutazone by ELISA 2013-05-15
CLG-TET2 Qualitative Identification of Tetracyclines 2011-09-19
CLG-TIL2 Confirmation of Tilmicosin by APCI-LC/MS3 2002-03-15
CLG-TRN Determination of Trenbolone by ELISA 2004-11-30
CLG-TRN1 Confirmation of α and β-Trenbolone by GC/MS/MS 2005-01-03
CLG-ZRL Screening for Zeranol by ELISA 2007-02-26

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Residue Chemistry (Inactive)

Method
Number
Method Title Effective
Date
CLG-CBX1 Determination of Carbadox Metabolite by GC/ECD 2010-03-24
CLG-CBX2 Confirmation of Carbadox Metabolite in Swine Liver by GC/EI Ion-trap MS 2005-08-22
CLG-CHC3 Determination of Chlorinated Hydrocarbons (CHCs) and Chlorinated Organophosphate Hydrocarbons (COPs) with Gel Permeation Chromatography (GPC) 2010-06-01
CLG-CLN3 ELISA Screening for β-Agonist Residues in Animal Retinal Tissue 2004-07-19
CLG-CYN Cyanide in Tissue, Processed Meats, and Serum 1991-07
CLG-DEX Dexamethasone 1991-07
CLG-DPN1 Determination of Dipyrone-Related Residues by HPLC 2004-10-25
CLG-EDB Ethylene Dibromide 1991-07
CLG-FET Fenthion 1991-07
CLG-FLX1 Determination of Flunixin in Cattle Liver by HPLC 2002-11-04
CLG-FLX2 Confirmation of Flunixin Residues in Bovine Liver by HPLC/ESI-MS/MS 2003-03-24
CLG-GAS Gasoline 1991-07
CLG-GEN Gentamicin in Porcine Tissue by HPLC 1991-07
CLG-HEC1 Screen for Halogenated Environmental Contaminants 2005-10-14
CLG-HLF Halofuginone 1991-07
CLG-LAS Lasalocid in Bovine Tissue 1991-07
CLG-LSD Lysergic Acid Diethylamine and Phencyclidine 1991-07
CLG-LVD Cyromazine and Melamine 1991-07
CLG-MEL1 Determination and Confirmation of Melamine by LC/MS/MS 2008-12-02
CLG-MGA1 Confirmation of Melengestrol Acetate by APCI/LC/MS3 2003-03-18
CLG-MGA2 Screen for Melengestrol Acetate in Fat Using ELISA 2006-02-01
CLG-MGA4 Determination of Melengestrol Acetate 2007-12-12
CLG-MON Monensin 1991-07
CLG-MPT Morantel and Pyrantel Drug-Related Metabolites 1991-07
CLG-NAR Narasin in Cattle and Chicken Tissues 1991-07
CLG-NBV Novobiocin and Virginiamycin 1991-07
CLG-NFUR1 Screen for Furazolidone and Furaltadone Metabolites by ELISA 2005-03-07
CLG-NIC Nicarbazin in Poultry Tissues 1991-07
CLG-NTR2 Nitrosamines/Low-Temperature Vacuum Distillation Procedure 1991-07
CLG-NTR3 Analysis of Nitrosamines using Supercritical Fluid Extraction 2009-06-30
CLG-PFC1 Determination and Confirmation of PFOA and PFOS by UPLC/MS/MS 2009-11-05
CLG-PST4 Confirmation of Pesticides by GC/MS/MS 2010-08-30
CLG-PYRE Synthetic Pyrethrins/c-ELISA 1991-07
CLG-RAC1 Determination of Ractopamine Hydrochloride by High Performance Liquid Chromatography 2013-05-03
CLG-SUL Determination and Confirmation of Sulfonamides 2009-09-25
CLG-SUL2 Automated Robotic Extraction/TLC Analysis for Sulfonamide Residues in Animal Tissues, Eggs, and Egg Products 2011-05-11
CLG-SUL3 Screening of Sulfonamides by Automated Robotic Extraction and Thin Layer Chromatography (TLC) 2012-02-24
CLG-TST2 Screening and Confirmation of Thyreostats by HPLC/MS/MS 2009-01-22

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Siluriformes Species Identification

Method
Number
Method Title Effective
Date
CLG-FPCR Fish Speciation by PCR/Sequencing of Mitochondrial Cytochrome Oxidase I (COI) Gene 2016-06-20

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Food Chemistry

Method
Number
Method Title Effective
Date
CLG-MOI Moisture Determination 2009-08-10
CLG-PRO4 Protein Determination by Combustion 2009-07-29
CLG-FAT Determination of Fat 2009-08-10
CLG-SLT Determination of Salt 2009-07-27
CLG-PHS1 Determination of Phosphate 2009-08-10
CLG-ICT1 Internal Cooking Temperature 1993-05
CLG-ICT2 Internal Cooking Temperature/Coagulation 1993-05

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Inorganic Chemistry

Method
Number
Method Title Effective
Date
CLG-TM3 Determination of Metals by ICP-MS and ICP-OES (Optical Emission Spectrometry) 2016-06-20
CLG-ARS Determination of Arsenic by Atomic Absorption Spectroscopy 2016-06-20
CLG-TM4 Detection of Thallium, Arsenic and Mercury using ICP-AES 2006-12-11
CLG-CAL Calcium/Tritimetric 1993-05
CLG-CAL2 Calcium, Inductively Coupled Plasma (ICP) Determination 2003-01-10
CLG-MER Determination of Mercury by Atomic Absorption Spectrophotometry 1991-07
CLG-MERC1 Determination of Mercury by Atomic Absorption Spectroscopy 2012-05-27
CLG-SFT1 Determination of Sulfites 2006-09-05
CLG-SOCAL3 Determination of Sodium and Calcium by ICP Atomic Emission Spectroscopy 2014-09-25

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All methods are offered as PDF documents. If you require an alternative format, please contact:

Chemistry Section
Laboratory Quality Assurance Staff, USDA/FSIS/OPHS
950 College Station Road
Athens, GA 30605 
Phone: (706) 546-3559 

 

Last Modified Jul 11, 2017