Chemistry Laboratory Guidebook
The Chemistry Laboratory Guidebook (CLG) contains test methods used by FSIS Field Service Laboratories to support FSIS regulatory activities. The CLG contains methods for the analysis of food composition, food additives, nutrients, veterinary drug and pesticide residues. Methods contained in the CLG are designed to provide FSIS analysts with written documentation to facilitate training, performance, quality assessment, and interpretation of data. FSIS does not specifically endorse any of the products mentioned in the CLG and acknowledges other products may be available for laboratory use.
Definitions for Chemistry Methods
Terms used in CLG methods are defined here
Allergen Verification | Food Chemistry | Inorganic Chemistry | Laboratory Introduction | Residue Chemistry (Inactive) | Residue Chemistry (Infrequent) | Residue Chemistry (Routine) | Siluriformes Species Identification
Laboratory Introduction
| Method Number |
Method Title | Effective Date |
|---|---|---|
| CLG-1 | FSIS Laboratory System Introduction, Method Performance Expectations, and Sample Handling for Chemistry | 2023-01-16 |
Residue Chemistry (Routine)
Allergen Verification
| Method Number |
Method Title | Effective Date |
|---|---|---|
| CLG-ALG1 |
Screening and Confirmation of Allergens by XMAP® Food Allergen Detection Assay |
2025-02-12 |
Food Chemistry
| Method Number |
Method Title | Effective Date |
|---|---|---|
| CLG-MOI |
|
2023-12-28 |
| CLG-PRO4 |
Quantitation of Protein by Combustion
|
2023-12-28 |
| CLG-FAT |
|
2023-08-28 |
| CLG-SLT |
|
2023-08-28 |
Inorganic Chemistry
| Method Number |
Method Title | Effective Date |
|---|---|---|
| CLG-TM3 | Determination of Metals by ICP-MS and ICP-OES (Optical Emission Spectrometry) | 2018-11-05 |
| CLG-TM4 | Detection of Thallium, Arsenic and Mercury using ICP-AES | 2006-12-11 |
| CLG-CAL | Calcium/Tritimetric | 1993-05 |
| CLG-CAL2 | Calcium, Inductively Coupled Plasma (ICP) Determination | 2003-01-10 |
| CLG-MER | Determination of Mercury by Atomic Absorption Spectrophotometry | 1991-07 |
| CLG-MERC1 | Determination of Mercury by Atomic Absorption Spectroscopy | 2018-04-13 |
| CLG-SOCAL3 | Determination of Sodium and Calcium by ICP Atomic Emission Spectroscopy | 2014-09-25 |
Siluriformes Species Identification
| Method Number |
Method Title | Effective Date |
|---|---|---|
| CLG-FPCR | Fish Speciation by PCR/Sequencing of Mitochondrial Cytochrome Oxidase I (COI) Gene | 2020-04-30 |
Infrequent Chemistry Methods
| Method Number |
Method Title | Effective Date |
|---|---|---|
| CLG-CEF2 | Quantitation and Confirmation of Desfuroylceftiofur Cysteine Disulfide by LC-MS-MS | 2020-12-07 |
| CLG-FLQ2 | Confirmation of Fluoroquinolone Antibiotics by HPLC Ion Trap Mass Spectrometry | 2021-04-26 |
| CLG-FLX4 | Determination and Confirmation of Flunixin by HPLC/ESI-MS/MS | 2021-03-15 |
| CLG-ICT1 | Internal Cooking Temperature | 1993-05 |
| CLG-ICT2 | Internal Cooking Temperature/Coagulation | 1993-05 |
| CLG-PENG1 | Determination and Confirmation of Penicillin G by LC-MS/MS | 2020-12-07 |
| CLG-PHS1 | Determination of Phosphate | 2009-08-10 |
| CLG-SFT1 | Determination of Sulfites | 2006-09-05 |
| CLG-SUL4 | Screening, Quantitation and Confirmation of Sulfonamides by Liquid Chromatography - Tandem Mass Spectrometry (LC-MS-MS) | 2021-02-01 |
| CLG-TIL1 | Determination of Tilmicosin by HPLC/Ion Pairing | 2021-03-15 |
Inactive Chemistry Methods
All methods are offered as PDF documents. If you require an alternative format, please contact:
Chemistry Section
Laboratory Quality Assurance Response and Coordination Staff (LQARCS), USDA/FSIS/OPHS
950 College Station Road
Athens, GA 30605
Phone: (706) 713-5898