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Web Content Viewer (JSR 286)

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Web Content Viewer (JSR 286)

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Web Content Viewer (JSR 286)

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Web Content Viewer (JSR 286)

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Microbiology Laboratory Guidebook

 
The Guidebook contains current protocols for analytical tests required by FSIS regulatory activities on meat, poultry and egg products. 1 Specifically, microbiological methods are presented for sample preparation, isolation and identification of the major foodborne pathogenic microorganisms and their toxins, meat tissue species identification, and the detection of antimicrobial residues. Appendices include a pathogen method summary chart, method flow charts, Media and Reagent formulations, and Most Probable Number Tables.
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Method Number Method Title
  Cover, Foreword, General Considerations (PDF Only)
1. Sample Preparation For Meat, Poultry and Pasteurized Egg Products (1998; PDF Only)
2. Physical Examination of Meat and Poultry Products (1998; PDF Only)
3.01 Quantitative Analysis of Bacteria in Foods as Sanitary Indicators (Jan 20, 2011; PDF Only)
4.08 Isolation and Identification of Salmonella from Meat, Poultry, Pasteurized Egg, and Catfish Products and Carcass and Environmental Sponges (Jun 29, 2014; PDF Only)
4C.06 FSIS Procedure for the Use of a Polymerase Chain Reaction (PCR) Assay for Screening Salmonella in Meat, Poultry, Pasteurized Egg, and Catfish Products and Carcass and Environmental Sponges (Jun 29, 2014; PDF Only)
5.08 Detection, Isolation and Identification of Escherichia coli O157:H7 from Meat Products and Carcass and Environmental Sponges (Jun 29, 2014; PDF Only)
5A.04 FSIS Procedure for the Use of Escherichia coli O157:H7 Screening Tests for Meat Products and Carcass and Environmental Sponges (Jun 29, 2014; PDF Only)
5B.05 Detection and Isolation of non-O157 Shiga Toxin-Producing Escherichia coli (STEC) from Meat Products and Carcass and Environmental Sponges (Jun 29, 2014; PDF Only)
7. Isolation and Identification of Aeromonas Species from Meat and Poultry Products (1998; PDF Only)
8.09 Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry and Egg Products, and Environmental Samples (May 1, 2013; PDF Only)
8A.05 FSIS Procedure for the Use of a Listeria monocytogenes Polymerase Chain Reaction (PCR) Screening Test (Nov 6, 2012; PDF Only)
9. Isolation & Identification of Pathogenic Yersinia enterocolitica from Meat and Poultry Products (1998; PDF Only)
10. Examination of Heat Processed, Hermetically Sealed (Canned) Meat and Poultry Products (1998; PDF Only)
11. Tests For Enzymes In Meat and Meat Products (1998; PDF Only)
12. Examination of Meat and Poultry Products For Bacillus cereus (1998; PDF Only)
13. Examination of Meat and Poultry Products For Clostridium perfringens (1998; PDF Only)
14. Methods for the Detection of Clostridium botulinum Toxins In Meat and Poultry Products (1998; PDF Only)
16. Agarose Thin-Layer Isoelectric Focusing (TLIEF) For Species Determination of Raw Muscle Tissues (1998; PDF Only)
17.02 Identification of Animal Species in Meat and Poultry Products (Feb 10, 2005; PDF Only)
18. Species Identification Field Tests (SIFT) (1998; PDF Only)
19. Competitive Enzyme-Linked Immunoassay (CELIA) For The Detection and Quantitation of Chloramphenicol (1998; PDF Only)
  Chapter Numbers 22-31 Reserved For Future Use.
34.03 Bioassay for the Detection, Identification and Quantitation of Antimicrobial Residues in Meat and Poultry Tissue (May 25, 2011; PDF Only)
40.00 Avian Influenza Detection in the Chicken Heart Using Real-Time Reverse Transcriptase PCR (Oct 8, 2007; PDF Only)
41.03 Isolation and Identification of Campylobacter jejuni/coli/lari from Poultry Rinse, Sponge and Raw Product Samples (Aug 18, 2014; PDF Only)
Appendices:
1.08 Media and Reagents (Oct 1, 2013; PDF Only)
2.05 Most Probable Number Procedure and Tables (Jun 29, 2014; PDF Only)
3.00 FSIS Laboratory Regulatory Sample Pathogen Methods Table and Definitions (Jun 29, 2014; PDF Only)
1 The methods described in this guidebook are for use by the FSIS laboratories. FSIS does not specifically endorse any of the mentioned test products and acknowledges that equivalent products may be available for laboratory use.

 

Last Modified Aug 04, 2014