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Chemistry Laboratory Guidebook

This Chemistry Laboratory Guidebook contains test methods used by FSIS Laboratories to support the Agency's inspection program, ensuring that meat, poultry, and egg products are safe, wholesome and accurately labeled.

The Guidebook contains methods for the analysis of food composition, food additives, nutrients, veterinary drug and pesticide residues. Methods are designed to provide analysts with written documentation to facilitate training, performance, quality assessment, and interpretation of data.

The contents of this Guidebook are continuously revised and updated. Future updates will include other methods previously published in the printed version of the Guidebook, which is no longer available for distribution.

Subscribe Receive email notification when the Chemistry Laboratory Guidebook is updated.

Note: All documents are PDF.

Method Number Method Title Effective Date
FOOD CHEMISTRY
F01 Moisture Determination Aug 10, 2009
F02 Protein Determination by Combustion Jul 27, 2009
F03 Determination of Fat Aug 10, 2009
F04 Determination of Salt Jul 27, 2009
F05 Determination of Phosphate Aug 10, 2009
F06 Internal Cooking Temperature May 1993
F07 Internal Cooking Temperature/Coagulation May 1993
INORGANIC CHEMISTRY
I01 Determination of Arsenic by Atomic Absorption Spectrophotometry Apr 2, 2010
I02a Calcium, Inductively Coupled Plasma (ICP) Determination Jan 10, 2003
I02b Calcium, Tritimetric Determination May 1993
I03 Determination of Metals by ICP-MS and ICP-OES (Optical Emission Spectrometry) Sep 30, 2013
I04 Detection of Thallium, Arsenic, and Mercury using ICP-AES Dec 11, 2006
I05a Determination of Mercury by Atomic Absorption Spectrophotometry Jul 1991
I05b Determination of Mercury by Atomic Absorption Spectroscopy May 27, 2012
I06 Determination of Sulfites Sep 5, 2006
I07 Determination of Sodium and Calcium by ICP-AES Sep 25, 2014
RESIDUE CHEMISTRY
R01a Confirmation of Aminoglycosides by HPLC-MS/MS Jun 3, 2011
R01b Screening and Confirmation for Aminoglycosides by LC-MS-MS Feb 27, 2013
R01c Screening for Aminoglycosides by LC-MS-MS Apr 10, 2014
R02a Screening for Zeranol by ELISA Feb 26, 2007
R02b Determination and Confirmation of Diethylstilbestrol (DES) and Zeranol by GC/MS Nov 10, 2004
R03a Determination of Ivermectin, Doramectin, and Moxidectin by HPLC Mar 28, 2011
R03b Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometric (LC/APCI/MS) Confirmation of Ivermectin, Doramectin and Moxidectin Mar 31, 2011
R04a ELISA Screening for β-Agonist Residues in Animal Retinal Tissue Jul 19, 2004
R04b Screening and Confirmation of Beta-Agonists by HPLC/MS/MS Apr 7, 2014
R05 Determination of Ractopamine Hydrochloride by High Performance Liquid Chromatography May 3, 2013
R06 Screening and Confirmation of β-Lactam Antibiotics by HPLC-MS/MS Jul 2, 2011
R07a Determination of Carbadox Metabolite by GC/ECD Mar 24, 2010
R07b Confirmation of Carbadox Metabolite in Swine Liver by GC/EI Ion-trap MS Aug 22, 2005
R07c Screen of Carbadox Metabolite QCA by HPLC-MS-MS Mar 28, 2011
R08a Screening for Chloramphenicol by ELISA Apr 15, 2013
R08b Determination and Confirmation of Chloramphenicol Sep 9, 2013
R09a Cyromazine and Melamine Jul 1991
R09b Determination and Confirmation of Melamine by LC/MS/MS (Ground Beef and Ready-to-Eat Products) Dec 2, 2008
R10 Dexamethasone Jul 1991
R11 Determination of Dipyrone-Related Residues by HPLC Oct 25, 2004
R12a Determination and Confirmation of Florfenicol Oct 12, 2010
R12b Confirmation of Florfenicol by GC/MS Aug 7, 2006
R13a Screening of Flunixin Residues by ELISA Mar 10, 2011
R13b Determination of Flunixin in Cattle Liver by HPLC Nov 4, 2002
R13c Determination and Confirmation of Flunixin by HPLC/ESI-MS/MS Jul 16, 2012
R13d Confirmation of Flunixin Residues in Bovine Liver by HPLC/ESI-MS/MS Mar 24, 2003
R14 Gentamicin in Porcine Tissue by HPLC Jul 1991
R15 Halofuginone Jul 1991
R16 Lasalocid in Bovine Tissue Jul 1991
R17 Lysergic Acid Diethylamine and Phencyclidine Jul 1991
R18 Confirmation of Macrolide/Lincosamide Antibiotics by Ion Trap HPLC/MS/MS Jul 30, 2007
R19a Screen for Melengestrol Acetate in Fat Using ELISA Feb 1, 2006
R19b Determination of Melengestrol Acetate Dec 12, 2007
R19c Confirmation of Melengestrol Acetate by APCI/LC/MS 3 Mar 18, 2003
R20 Monensin Jul 1991
R21 Morantel and Pyrantel Drug-Related Metabolites Jul 1991
R22 Narasin in Cattle and Chicken Tissues Jul 1991
R23a Screen for Furazolidone and Furaltadone Metabolites by ELISA Mar 7, 2005
R23b Screening and Confirmation of Nitrofuran Metabolites by Liquid Chromatography-Tandem Mass Spectrometry Mar 9, 2010
R23c Screening and Confirmation of Nitrofuran Metabolites in Poultry Muscle by Liquid Chromatography-Tandem Mass Spectrometry Aug 1, 2014
R24a Screen for Nitroimidazoles by HPLC Dec 20, 2005
R24b Confirmation of Nitroimidazoles by ESI-LC/MS/MS Feb 7, 2005
R25 Novobiocin and Virginiamycin Jul 1991
R26a Screening of Phenylbutazone Residues by ELISA May 15, 2013
R26b Confirmation of Phenylbutazone in Bovine Kidney by LC/ESI-MS/MS Dec 10, 2007
R27a Determination and Confirmation of Sulfonamides Sep 25, 2009
R27b Automated Robotic Extraction - TLC Analysis for Sulfonamide Residues in Animal Tissues, Eggs, and Egg Products May 11, 2011
R27c Screening of Sulfonamides by Automated Robotic Extraction and Thin Layer Chromatography (TLC) Feb 24, 2012
R27d Quantitation and Confirmation of Sulfonamides by Liquid Chromatography - Tandem Mass Spectrometry (LC-MS-MS) Apr 22, 2013
R28 Qualitative Identification of Tetracyclines in Tissues Sep 19, 2011
R29 Screening and Confirmation of Thyreostats by HPLC/MS/MS Jan 22, 2009
R30a Determination of Tilmicosin by HPLC/Ion Pairing Aug 25, 2005
R30b Confirmation of Tilmicosin by APCI-LC/MS 3 Mar 15, 2002
R31a Determination of Trenbolone by ELISA Nov 30, 2004
R31b Confirmation of α and β-Trenbolone by GC/MS/MS Jan 3, 2005
R32 Confirmation of Fluoroquinolone Antibiotics by HPLC Ion Trap Mass Spectrometry Jan 13, 2006
R33a Screen for Halogenated Environmental Contaminants Oct 14, 2005
R33b Determination of Chlorinated Hydrocarbons (CHCs) and Chlorinated Organophosphate Hydrocarbons (COPs) with Gel Permeation Chromatography (GPC) Jun 1, 2010
R33c Confirmation of Pesticides by GC/MS/MS Aug 30, 2010
R33d Screening for Pesticides by LC/MS/MS and GC/MS/MS Jul 6, 2014
R34 Cyanide in Tissue, Processed Meats, and Serum Jul 1991
R35 Ethylene Dibromide Jul 1991
R36 Fenthion Jul 1991
R37 Gasoline Jul 1991
R38a Analysis of Nitrosamines using Supercritical Fluid Extraction Jun 30, 2009
R38b Nitrosamines/Low-Temperature Vacuum Distillation Procedure Jul 1991
R39 Synthetic Pyrethrins/c-ELISA Jul 1991
R40 Determination of Benzoic Acid, Sorbic Acid, and Methyl, Ethyl, Propyl, and Butyl Parabens by HPLC Nov 1, 2004
R41 Inhibition Screen Test for Antimicrobial Drugs Aug 19, 2012
R42 Determination and Confirmation of PFOA and PFOS by UPLC/MS/MS Nov 5, 2009
R43 Nicarbazin in Poultry Tissues Jul 1991
R44a Screening for Malachite Green and Crystal Violet by ELISA Apr 8, 2010
R44b Confirmation of Malachite Green and Crystal Violet by UHPLC-MS-MS Oct 13, 2010
R45 Screening and Confirmation of Animal Drug Residues by UHPLC-MS-MS (&quotMulti-Residue Method&quot) Feb 10, 2014
R46 Determination and Confirmation of Penicillin G by LC-MS/MS Aug 24, 2012
R47 Determination for Ceftiofur-Related (DCA) Residues by HPLC Oct 1, 2012
R48 Identification of Poisons and Toxins by GC-MS May 5, 2013
R49 Determination of Multiple Hormones in Beef Muscle by LC/MS/MS Sep 15, 2014
SPECIATION
S01 Fish Speciation by Isoelectric Focusing Dec 7, 2009
S02 Fish Speciation by PCR/Sequencing of Mitochondrial Cytochrome Oxidase I (COI) Gene Mar 9, 2012

Accessibility Note: All methods are offered as PDF documents. Due to age, a few documents may be searchable but not fully compliant with Section 508. If you require an alternative format, please contact:

Chemistry Branch
Quality Assurance Division, USDA/FSIS/OPHS
950 College Station Road
Athens, GA 30605

 

Last Modified Sep 30, 2014