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| Chemistry Laboratory Guidebook
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This Chemistry Laboratory Guidebook
contains test methods used by FSIS Laboratories to support the Agency's
inspection program, ensuring that meat, poultry, and egg products
are safe, wholesome and accurately labeled.
The Guidebook contains
methods for the analysis of food composition, food additives, nutrients,
veterinary drug and pesticide residues. Methods are designed to provide
analysts with written documentation to facilitate training, performance,
quality assessment, and interpretation of data.
The contents of this Guidebook are continuously revised and updated.
Future updates will include other methods previously published in
the printed version of the Guidebook, which is no longer available
for distribution. |
Receive email notification when the Chemistry Laboratory Guidebook is updated.
|
Methods
Available On This Site:
| Method Number |
Method Title |
Effective Date |
| FOOD CHEMISTRY |
| F01 |
Moisture
Determination (PDF Only) |
Aug 10, 2009 |
| F02 |
Protein
Determination by Combustion (PDF Only) |
Jul 27, 2009 |
| F03 |
Determination of Fat (PDF Only) |
Aug 10, 2009 |
| F04 |
Determination of Salt (PDF Only) |
Jul 27, 2009 |
| F05 |
Determination of Phosphate (PDF Only) |
Aug 10, 2009 |
| F06 |
Internal Cooking Temperature (PDF Only) |
May 1993 |
| F07 |
Internal Cooking Temperature/Coagulation (PDF Only) |
May 1993 |
| INORGANIC CHEMISTRY |
| I01 |
Determination of Arsenic by Atomic Absorption Spectrophotometry
(PDF Only) |
Apr 2, 2010 |
| I02a |
Calcium,
Inductively Coupled Plasma (ICP) Determination (PDF Only) |
Jan 10, 2003 |
| I02b |
Calcium, Tritimetric Determination (PDF Only) |
May 1993 |
| I03 |
Determination of Metals by ICP-MS (PDF Only) |
Aug 24, 2012 |
| I04 |
Detection
of Thallium, Arsenic, and Mercury using ICP-AES (PDF Only) |
Dec 11, 2006 |
| I05a |
Determination
of Mercury by Atomic Absorption Spectrophotometry (PDF Only) |
Jul 1991 |
| I05b |
Determination of Mercury by Atomic Absorption Spectroscopy (PDF Only) |
May 27, 2012 |
| I06 |
Determination of
Sulfites (PDF Only) |
Sep 5, 2006 |
| RESIDUE CHEMISTRY |
| R01a |
Confirmation
of Aminoglycosides by HPLC-MS/MS (PDF Only) |
Jun 3, 2011 |
| R01b |
Screening and Confirmation for Aminoglycosides by LC-MS-MS (PDF Only) |
Dec 21, 2012 |
| R02a |
Screening
for Zeranol by ELISA (PDF Only) |
Feb 26, 2007 |
| R02b |
Determination
and Confirmation of Diethylstilbestrol (DES) and Zeranol by
GC/MS (PDF Only) |
Nov 10, 2004 |
| R03a |
Determination of Ivermectin, Doramectin,
and Moxidectin by HPLC (PDF Only) |
Mar 28, 2011 |
| R03b |
Liquid Chromatography/Atmospheric
Pressure Chemical Ionization Mass Spectrometric (LC/APCI/MS) Confirmation of Ivermectin, Doramectin and Moxidectin (PDF Only) |
Mar 31, 2011 |
| R04a |
ELISA
Screening for β-Agonist Residues in Animal Retinal Tissue
(PDF Only) |
Jul 19, 2004 |
| R04b |
Screening and Confirmation of Beta-Agonists by HPLC/MS/MS
(PDF Only) |
Jul 2, 2012 |
| R05 |
Determination
of Ractopamine Hydrochloride by High Performance Liquid Chromatography
(PDF Only) |
Jul 6, 2004 |
| R06 |
Screening
and Confirmation of β-Lactam Antibiotics by HPLC-MS/MS (PDF Only) |
Jul 2, 2011 |
| R07a |
Determination
of Carbadox Metabolite by GC/ECD (PDF Only) |
Mar 24, 2010 |
| R07b |
Confirmation
of Carbadox Metabolite in Swine Liver by GC/EI Ion-trap MS
(PDF Only) |
Aug 22, 2005 |
| R07c |
Screen of Carbadox Metabolite QCA by HPLC-MS-MS
(PDF Only) |
Mar 28, 2011 |
| R08a |
Screening for
Chloramphenicol by ELISA (PDF Only) |
Jul 23, 2012 |
| R08b |
Determination and Confirmation of Chloramphenicol (PDF Only) |
Feb 1, 2013 |
| R09a |
Cyromazine
and Melamine (PDF Only) |
Jul 1991 |
| R09b |
Determination and Confirmation of Melamine by LC/MS/MS (Ground Beef and Ready-to-Eat Products) (PDF Only, 1.6mb) |
Dec 2, 2008 |
| R10 |
Dexamethasone
(PDF Only) |
Jul 1991 |
| R11 |
Determination
of Dipyrone-Related Residues by HPLC (PDF
Only |
Oct 25, 2004 |
| R12a |
Determination
and Confirmation of Florfenicol (PDF Only) |
Oct 12, 2010 |
| R12b |
Confirmation
of Florfenicol by GC/MS (PDF Only) |
Aug 7, 2006 |
| R13a |
Screening of Flunixin Residues by ELISA (PDF Only) |
Mar 10, 2011 |
| R13b |
Determination
of Flunixin in Cattle Liver by HPLC (PDF
Only) |
Nov 4, 2002 |
| R13c |
Determination and Confirmation of Flunixin by HPLC/ESI-MS/MS (PDF Only) |
Jul 16, 2012 |
| R13d |
Confirmation
of Flunixin Residues in Bovine Liver by HPLC/ESI-MS/MS
(PDF Only) |
Mar 24, 2003 |
| R14 |
Gentamicin
in Porcine Tissue by HPLC (PDF Only) |
Jul 1991 |
| R15 |
Halofuginone
(PDF Only) |
Jul 1991 |
| R16 |
Lasalocid
in Bovine Tissue (PDF Only) |
Jul 1991 |
| R17 |
Lysergic
Acid Diethylamine and Phencyclidine (PDF Only) |
Jul 1991 |
| R18 |
Confirmation
of Macrolide/Lincosamide Antibiotics by Ion Trap HPLC/MS/MS
(PDF Only) |
Jul 30, 2007 |
| R19a |
Screen
for Melengestrol Acetate in Fat Using ELISA (PDF Only) |
Feb 1, 2006 |
| R19b |
Determination
of Melengestrol Acetate (PDF
Only) |
Dec 12, 2007 |
| R19c |
Confirmation
of Melengestrol Acetate by APCI/LC/MS3 (PDF Only) |
Mar
18, 2003 |
| R20 |
Monensin
(PDF Only) |
Jul 1991 |
| R21 |
Morantel
and Pyrantel Drug-Related Metabolites (PDF Only) |
Jul 1991 |
| R22 |
Narasin
in Cattle and Chicken Tissues (PDF Only) |
Jul 1991 |
| R23a |
Screen
for Furazolidone and Furaltadone Metabolites by ELISA
(PDF Only) |
Mar 7, 2005 |
| R23b |
Screening and Confirmation of
Nitrofuran Metabolites by Liquid Chromatography-Tandem Mass Spectrometry
(PDF Only) |
Mar 9, 2010 |
| R24a |
Screen
for Nitroimidazoles by HPLC (PDF Only) |
Dec 20, 2005 |
| R24b |
Confirmation
of Nitroimidazoles by ESI-LC/MS/MS (PDF Only) |
Feb 7, 2005 |
| R25 |
Novobiocin
and Virginiamycin (PDF Only) |
Jul 1991 |
| R26a |
Screening of Phenylbutazone
Residues in Bovine Kidney by ELISA (PDF Only) |
Aug 18, 2008 |
| R26b |
Confirmation
of Phenylbutazone in Bovine Kidney by LC/ESI-MS/MS (PDF Only) |
Dec 10, 2007 |
| R27a |
Determination
and Confirmation of Sulfonamides (PDF Only) |
Sep 25, 2009 |
| R27b |
Automated Robotic Extraction – TLC Analysis for
Sulfonamide Residues in Animal Tissues, Eggs, and Egg Products (PDF Only) |
May 11, 2011 |
| R27c |
Screening of
Sulfonamides by Automated Robotic Extraction and Thin Layer Chromatography (TLC) (PDF Only) |
Feb 24, 2012 |
| R27d |
Quantitation and Confirmation of Sulfonamides
by Liquid Chromatography – Tandem Mass Spectrometry (LC-MS-MS) (PDF Only) |
Apr 22, 2013 |
| R28 |
Qualitative Identification of
Tetracyclines in Tissues (PDF Only) |
Sep 19, 2011 |
| R29 |
Screening and Confirmation
of Thyreostats by HPLC/MS/MS (PDF Only) |
Jan 22, 2009 |
| R30a |
Determination
of Tilmicosin by HPLC/Ion Pairing (PDF Only) |
Aug 25, 2005 |
| R30b |
Confirmation
of Tilmicosin by APCI-LC/MS3 (PDF Only) |
Mar 15, 2002 |
| R31a |
Determination
of Trenbolone by ELISA (PDF Only) |
Nov 30, 2004 |
| R31b |
Confirmation
of α and β-Trenbolone by GC/MS/MS (PDF Only) |
Jan 3, 2005 |
| R32 |
Confirmation
of Fluoroquinolone Antibiotics by HPLC Ion Trap Mass Spectrometry
(PDF Only) |
Jan 13, 2006 |
| R33a |
Screen
for Halogenated Environmental Contaminants (PDF Only) |
Oct 14, 2005 |
| R33b |
Determination of Chlorinated Hydrocarbons
(CHCs) and Chlorinated Organophosphate Hydrocarbons (COPs) with Gel Permeation Chromatography (GPC) (PDF Only) |
Jun 1, 2010 |
| R33c |
Confirmation of
Pesticides by GC/MS/MS (PDF Only; 2.2mb) |
Aug 30, 2010 |
| R33d |
Screening for Pesticides by LC/MS/MS and GC/MS/MS (PDF Only; 4.2mb) |
Aug 6, 2012 |
| R34 |
Cyanide
in Tissue, Processed Meats, and Serum (PDF Only) |
Jul 1991 |
| R35 |
Ethylene
Dibromide (PDF Only) |
Jul 1991 |
| R36 |
Fenthion
(PDF Only) |
Jul 1991 |
| R37 |
Gasoline
(PDF Only) |
Jul 1991 |
| R38a |
Analysis
of Nitrosamines using Supercritical Fluid Extraction (PDF Only) |
Jun 30, 2009 |
| R38b |
Nitrosamines/Low-Temperature Vacuum Distillation Procedure (PDF Only; 1.2mb) |
Jul 1991 |
| R39 |
Synthetic
Pyrethrins/c-ELISA (PDF Only) |
Jul 1991 |
| R40 |
Determination
of Benzoic Acid, Sorbic Acid, and Methyl, Ethyl, Propyl, and
Butyl Parabens by HPLC (PDF Only) |
Nov 1, 2004 |
| R41 |
Inhibition Screen Test for Antimicrobial Drugs
(PDF Only) |
Aug 19, 2012 |
| R42 |
Determination and Confirmation of PFOA and PFOS by UPLC/MS/MS (PDF Only) |
Nov 5, 2009 |
| R43 |
Nicarbazin in Poultry Tissues (PDF Only; 1.2mb) |
Jul 1991 |
| R44a |
Screening for Malachite Green and Crystal Violet by ELISA (PDF Only) |
Apr 8, 2010 |
| R44b |
Confirmation of
Malachite Green and Crystal Violet by UHPLC-MS-MS (PDF Only; 1.2mb) |
Oct 13, 2010 |
| R45 |
Screening and Confirmation of Animal Drug Residues by UHPLC-MS-MS
(PDF Only) |
Feb 22, 2013 |
| R46 |
Determination and Confirmation of Penicillin G by LC-MS/MS
(PDF Only) |
Aug 24, 2012 |
| R47 |
Determination for Ceftiofur-Related (DCA) Residues by HPLC
(PDF Only) |
Oct 1, 2012 |
| SPECIATION |
| S01 |
Fish Speciation by Isoelectric Focusing (PDF Only) |
Dec 7, 2009 |
| S02 |
Fish Speciation by PCR/Sequencing of Mitochondrial Cytochrome Oxidase I (COI) Gene (PDF Only) |
Mar 9, 2012 |
|
Accessibility Note: All methods are offered as PDF documents. Due to age, a few
documents may be searchable but not fully compliant with Section 508.
If you require an alternative format, please contact:
Chemistry Branch
Quality Assurance Division, USDA/FSIS/OPHS
950 College Station Road
Athens, GA 30605 |
Last Modified: May 13, 2013 |
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