Method Description

LDL/ MIC

MPL

Species/ Tissues

Reference

HCB

Micro alumina assay: column chromatography plus GLC

0.01 ppm

NE

All/fat

1

Mills method: Florisil column chromatography plus GLC  

0.01 ppm

0.10 ppm

All/fat,PP

1

Extracts from GPC or Mills are confirmed by GC/ MS

0.01 ppm

NE

All/fat,PP

1

Heptachlor and heptachlor epoxide

Micro alumina assay: column chromatography plus GLC

0.01 ppm

NE

All/fat, PP

1

Mills method: Florisil column chromatography plus GLC

0.01 ppm

0.10 ppm

All/fat, PP

1

Extracts from GPC or Mills are confirmed by GC/MS

0.02 ppm

NE

All/fat,pp

1

Hetacillin, Potassium

Antibiotic screen test (Swab): ability of tissue fluids containing anti- microbial activity to inhibit microbial growth

NE

NE

All/kidney

16

5-Hydroxy- thiabendazole

Extraction with organic solvents followed by HPLC withUV detection; extracts confirmed by derivatized GC/EI/MS

0.05 ppm

NE

Red meat/ liver, muscle

14

Hygromycin B

Antibiotic screen test (Swab):ability of tissue fluids containing antimicrobial activity toinhibit microbial growth

5.00 ppm

NE

All/kidney

16

Ipronidazole and hydroxy metabolite

Tissue extracts are quantified by HPLC/UV

1.0 ppb

NE

Turkey, swine/ muscle

14

Tissue extracts from HPLC are confirmed by GC/NICI/MS

1.0 ppb

NE

Turkey, swine muscle

14

Iron

Dry ashed tissue is dissolved and quantified by AAS or by inductively coupled plasma

All/kidney, liver, muscle

1

Method Description

LDL/ MIC

MPL

Species/ Tissues

Reference

Ivermectin

Tissue extracts are quantified by HPLC fluorescence

2 ppb

5 ppb

Red meat/ liver, muscle

1

Derivatization to form 3 components with detection by HPLC fluorescence

2 ppb

NE

Red meat/ liver, muscle

1

Lasalocid

Tissue extracts are quantified by HPLC fluorescence detector

0.025 ppm

NE

Poultry/fat, skin

24

Tissue extraction followed by bioautography

0.005 ppm

0.01 ppm

Poultry/fat, skin

24

GC pyrolysis of the HPLCextract with MS identification of the fragments

0.2 ppm

NE

Cattle/liver; Poultry/fat, skin

24

Lead

Dry ashed tissue is dissolved and quantified by AAS or by inductively coupled plasma

0.05 ppm

NE

All/kidney liver, muscle

Dry ashed tissue is quantified by anodic stripping voltammetry

1.0 ppb

NE

Poultry/ kidney, liver

7

Levamisole

Tissue extracts are quantified by GLC flame photometric detection

0.05 ppm

NE

Red meat/ liver, muscle

1

Tissue extracts are subjected to GC/MS

0.05 ppm

NE

Red meat/ liver, muscle

1

Lincomycin hydrochloride

Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity toinhibit microbial growth

0.10 ppm

0.10 ppm

Poultry, swine/all

25

Lindane

Micro alumina assay: column chromatography plus GLC

0.01 ppm

NE

All/fat,PP

1

Mills method: Florisil column chromatography plus GLC

0.01 ppm

0.10 ppm

All/fat,PP

1

Extracts from GPC or Mills are confirmed by GC/MS

0.01 ppm

NE

All/fat,PP

1

Linuron

Extracts are confirmed by GC/EI/MS

0.05 ppm

NE

Red meat/ fat

1

Method Description

LDL/ MIC

MPL

Species/ Tissues

Reference

Lysergic acid diethylamide

Tissue extracts are spotted forTLC and detected with specific chromagenic reagent

NE

NE

All/kidney, liver, muscle

1

Tissue extracts are quantified by GLC with flame photometric muscle or nitrogen-phosphorous flame ionization detector

0.10 ppm

NE

All/liver,

1

Manganese

Dry ashed tissue is dissolved and quantified by AAS or by inductively coupled plasma

0.001 ppb

NE

All/kidney, liver, muscle

1

Mebendazole

Extraction with organic solvents followed by HPLC with UV detection; extracts are confirmed by GC/EI/MS

0.05 ppm

NE

Red meat/ liver, muscle

1

Melengestrol acetate (MGA)

Tissue extract is column chromatographed on Florisil and quantified by GLC

5.0 ppb

10.0 ppb

Cattle/liver muscle, kidney, fat

1

Extracts are derivatized with HFB and confirmed by GC/EI/MS

5.0 ppb

10.0 ppb

Cattle/fat

1

Mercury

Tissue is digested in acid. Mercury is reduced to its vapor and quanti- fied by flameless AAS

0.01 ppm

0.02 ppm

All/kidney, liver, muscle

1

Methanearsonic acid

Dry ashed tissue is dissolved and reacted to produce arsine liver, muscle gas, which is quantified by AAS

0.05 ppm

NE

All/kidney,

1

Dry ashed tissue is dissolved and reacted to produce arsine gas,which reacts to form blue complex for colorimetric quantification

0.05 ppm

0.20 ppm

All/kidney, liver, muscle

1

Methomyl

GPC plus HPLC with post- column fluorescence detection

5 ppb

10 ppb

All/liver

1

Methoxychlor

GPC plus GLC

0.15 ppm

0.50 ppm

All/fat

1

Extracts from GPC or Mills are confirmed by GC/MS

0.15 ppm

NE

All/fat,

1

Methyl parathion

Tissue extracts are quantified by GLC with flame photometric or nitrogen-phosphorous flame ionization detector

0.10 ppm

NE

All/liver, muscle

1

Monensin

Tissue extract is partitioned by TLC and semi-quantified by inhibition of micro-organism growth

0.05 ppm

0.10 ppm

Cattle, poultry/ liver ,fat

1

Method Description

LDL/ MIC

MPL

Species/ Tissues

Reference

Morantel tartrate

Tissue extract is hydrolyzed and a derivative is quantified by GLC

0.50 ppm

NE

Cattle/muscle

1

Identification of a structurally significant hydrolyzed fragment by GC/MS

0. 25 ppm

NE

Cattle/liver, muscle

26

Narasin

Tissue extracts are spotted on TLC and quantified with a bio-autographic overlay

5 ppb

NE

Cattle, poultry/ liver, kidney, fat

1

Neomycin

Tissue is deproteinized, acidified, and extracted; quantified by HPLC/UV

1.5 ppm

NE

All Kidney

14

 Antibiotic screen test (Swab):ability of tissue fluids containing anti-microbial activity to inhibit microbial growth

0.25 ppm

NE

All/kidney

16

Tissue extracts are screened by E-Z Screen

5.0 ppb

NE

All/muscle/ liver, kidney

10

Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity to inhibit microbial growth

0.25

NE

All/kidney, liver, muscle

27

Nequinate

Zymark Pytechnology System: Tissue extracts are screened by HPLC/UV

NE

NE

Cattle/liver, muscle

14

Nicarbazin

Tissues are extracted with ethyl acetate; the dinitrocarbanilide moiety isquantified by HPLC with a UV detector.Extracts verified by photodiode array detection

0.1 ppm

2.0 ppm

Chicken/ all tissues

1

Nickel

Dry ashed tissue is dissolved and quantified by AAS or by inductively coupled plasma.

NE

0.34 ppb

All/kidney, liver, muscle

1

Nonachlor

GPC plus GLC

0.03 ppm

0.15 ppm

All/fat

1

Mills method: Florisil column chromatography plus GLC

0.05 ppm

0.15 ppm

All/fat,PP

1

Extracts from GPC or Mills are confirmed by GC/MS

0.05 ppm

NE

All/fat, PP

1

Novobiocin

Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity to inhibit microbial growth

0.125 ppm

NE

All/kidney, liver, muscle

4

Zymark Pytechnology System: organic solvent extraction followed by HPLC/UV detection.

0.50 ppm

NE

All/kidney, liver, muscle

14

Manual system organic solvent extraction followed by HPLC/UV detection

0.50 ppm

NE

All/kidney, liver, muscle

1

Method Description

LDL/ MIC

MPL

Species/ Tissues

Reference

Oleandomycin

Antibiotic screen test (Swab): ability of tissue fluids containing antimicrobial activity to inhibit microbial growth

0.25 ppm

NE

All/kidney,

16

Oxfendazole

Extraction with organic solvents followed by HPLC with UV detection; extracts are derivatized and confirmed by GC/EI/MS

0.05 ppm

NE

Red meat, liver, muscle, PP

1

Oxytetracycline hydrochloride

Antibiotic screen test (Swab): ability of tissue fluids containing anti-microbial activity to inhibit microbial growth

0.08 ppm

NE

All/kidney

16

Microbiological assay procedure: ability of tissue extracts containing Lab antimicrobial activity to inhibit microbial growth

0.08 ppm

0.08 ppm

All/kidney, liver muscle

Sec. 6.312

Extraction using C18 columns followed by HPLC with UV detection

0.15 ppm

0.25 ppm

All/kidney, liver muscle

Parathion

Tissue extracts are quantified by GLC with flame photometric ornitrogen-phosphorous flame ionization detector

NE

NE

All/liver, muscle

14

PBB's

Micro alumina assay: column chromatography plus GLC detection by electron capture. UV degradation of PBB's is used as confirmation.

0.05 ppm

NE

All/fat

29

PCB's (reported as Aroclor 1242, 1248, 1254, 1260, etc.)

GPC plus GLC

0.30 ppm

0.50 ppm

All/fat

1

 Penicillin, procaine and procaine G

Antibiotic screen test (Swab): ability of tissue containing anti-microbial activity to inhibit microbial growth.

12.5 ppb

NE

All/kidney

16

(benzathine, free acid, sodium salt, and procaine salts)

Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity to inhibit microbial growth

12.5 ppb

NE

All/kidney, liver muscle

30

Pentachloroanisole

GPC plus GC Extracts confirmed by GC/EI/MS

NE

NE

Poultry/fat

14

Tissue digestate is extracted with cyclo- hexane and quantified by GLC

0.03 ppm

0.05 ppm

All/liver, muscle

1

Extracts confirmed by GC/EI/MS

NE

NE

Poultry/fat

1

(cis & trans)

Organic solvent extracts are quantified as the sum of both isomers; extracts are confirmed by GC/EI/MS

1.0 ppb

NE

All/fat

1

Phencyclidine

Tissue extracts are spotted for TLC with specific chromogenic agent

NE

NE

All/liver, muscle

1

Phenotrin

Solvent extraction followed by a competitive ELISA determination

1.0 ppm

NE

All/fat

1

Phosalone

GPC extracts are confirmed by GC/EI/MS

0.05 ppm

NE

Red meat, fat

1

Method Description

LDL/ MIC

MPL

Species/ Tissues

Reference

Propazine

Extracts confirmed by GC/MS

5 ppb

NE

All/fat

1

Promecarb

GPC plus HPLC with post- column flourescence detection. Extracts are subjected to reverse phase chromatography, derivativized and confirmed by GC/MS

5 ppb

10 ppb

All/liver

1

Propoxur

GPC plus HPLC with post- column fluorescence detection. Extracts are subjected to reverse phase chromatography, derivatitized and confirmed by GC/MS

5 ppb

10 ppb

All/liver

1

Pyrantel tartrate

Tissue extract is hydrolyzed and a derivative is quantified by GLC

0.25 ppm

0.50 ppm

Swine/liver, muscle

1

Identification of a structurally significant hydrolyzed fragment by GC/MS

0.25 ppm

NE

Swine/liver, muscle

31

Ronnel

GPC plus GLC

0.02 ppm

0.05 ppm

All/fat

1

0.10 ppm (red meat)

NE

Poultry, red meat, fat

1

Roxarsone

Dry ashed tissue is dissolved and reacted to produce arsinegas, which is quantified by AAS. Dry ashed tissue is dissolved and reacted to produce arsine liver muscle gas, which reacts to form blue complex for colorimetric quantification 

0.05 ppm

0.20 ppm

All/kidney

1

Simazine

Extracts confirmed by GC/MS

5 ppb

NE

All/fat

1

Spectinomycin hydrochloride

Microbiological assay: tissue extracts are quantified using a turbidimetric assay.

2.8 ppm

NE

All/kidney, liver, muscle

32

Streptomycin

0.25 ppm

NE

All/kidney, liver, muscle

35

Tissues are subjected to GC/MS head space analysis.

1 ppb

NE

All/kidney, liver, muscle fat, pp

1

Sulfachloro- pyridazine

TLC fluorescence: tissue extracts are partitioned by TLC and quantified by densitometry

0.02 ppm

0.05 ppm

Red meat/ liver, muscle

1

Extraction followed by GC/CI and EI/MS

0.05 ppm

NE

Red meat/ liver, muscle

1

Sulfadiazine

TLC fluorescence: tissue extracts are partitioned by TLC and quantified by densitometry

0.02 ppm

0.05 ppm

Red meat/liver, muscle

1

Sulfadimethoxine

TLC fluorescence: tissue extracts are partitioned by TLC and quantified by densitometry

0.02 ppm

0.05 ppm

All/liver, muscle

1

Tissue extracts are screened by E-Z Screen

50 ppb

NE

All/liver

10

Sulfadoxine

TLC fluorescence: tissue extracts are partitioned by TLC and quantified by densitometry.

0.05 ppm

NE

Red meat/ liver, muscle

1

Tissue extracts are confirmed by GC/EI/MS

NE

NE

Red meat/ liver, muscle

1

Sulfaethoxy- pyridazine

TLC fluorescence: tissue extracts are partitioned by TLC and quantified by densitometry

0.02 ppm

0.05 ppm

Red meat/ liver, muscle

1

Method Description

LDL/ MIC

MPL

Species/ Tissues

Reference

Sulfamethazine

TLC fluorescence: tissue extracts are partitioned by TLC and quantified by densitometry.

0.02 ppm

0.05 ppm

All/liver, muscle; Red meat/feed concentrate pp

1

Tissue extracts are confirmed by GC/EI/MS

0.05 ppm

NE

All/liver, muscle, pp

1

Tissue extracts are detected by TLC fluorescence (SOS-urine)

NE

NE

Swine/urine

14

Tissue extracts are screened by E-Z Screen

50 ppb

NE

All/liver

1

Sulfamethoxy- pyridazine

TLC fluorescence: tissue extracts are partitioned by TLC and quantified by densitometry

0.02 ppm

0.05 ppm

Red meat/ liver, muscle

1

Sulfaphenazole

TLC fluorescence: tissue extracts are partitioned by TLC and quantified by densitometry

0.05 ppm

NE

Red meat/ liver, muscle

1

Tissue extracts are confirmed by GC/EI/MS

NE

NE

Red meat/ liver, muscle

1

Sulfapyridine

TLC fluorescence: tissue extracts are partitioned by TLC and quantified by densitometry.

0.02 ppm

0.05 ppm

All/liver, muscle

1

Tissue extracts are confirmed by GC/EI/MS

0.05

NE

All/liver, muscle, pp

1

Method Description

LDL/ MIC

MPL

Species/ Tissues

Reference

Sulfaquinoxaline

TLC fluorescence: tissue extracts are partitioned by TLC and quantified by densitometry.

12.5 ppb

25.0 ppb

Poultry/liver, muscle

1

Tissue extracts are confirmed by GC/EI/MS

25 ppb

NE

Poultry/liver, muscle

1

Sulfathiazole

Tissue extracts are confirmed by GC/EI/MS 

0.05 ppm

NE

Red meat/ liver, muscle, pp

1

Sulfatroxazole

Tissue extracts are confirmed by GC/EI/MS

NE

NE

Red meat/ liver, muscle

1

Tissue extracts are confirmed by GC/EI/MS

NE

NE

Red meat/ liver, muscle

1

Method Description

LDL/ MIC

MPL

Species/ Tissues

Reference

Sulfisoxazole

TLC fluorescence: tissue extracts are partitioned by TLC and quantified by densitometry

0.05 ppm

NE

Red meat/liver, muscle

1

Tissue extracts are confirmed by GC/EI/MS

Tetrametrin

Solvent extraction followed by a competitive ELISA determination

1.0 ppm

NE

All/fat

1

(metabolite of DDT reported as DDT)

GPC plus GLC

0.03 ppm

0.15 ppm

All/fat

1

Extracts from GPC or Mills are confirmed by GC/MS

0.02 ppm

NE

All/fat pp

1

Terbuthylazine

5 ppb

NE

All/fat

1

Extracts confirmed by GC/MS

5 ppb

NE

All/fat

1

Terpene polychlorinates

Micro alumina assay: column chromatography plus GLC

0.50 ppm

NE

All/fat

1

Mills method: Florisil column chromatography plus GLC

0.50 ppm

NE

All/fat

1

Tetracycline hydrochloride

Antibiotic screen test (Swab): ability of tissue chloride fluids containing anti-microbial activity to inhibit microbial growth

0.08 ppm

NE

All/kidney

16

 Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity to inhibit microbial growth.

0.08 ppm

NE

All/kidney, liver, muscle

34

Extraction using C18 columns followed by HPLC with UV detection.

0.15 ppm

0.25 ppm

All/kidney, liver, muscle

14

Thiabendazole

pH extraction with organic solvents followed by HPLC with UV detection; extracts derivatized and confirmed by GC/EI/MS

0.05 ppm

NE

Red meat, pp liver, muscle

1

Extracts confirmed by GC/MS

NE

0.4 ppm

Swine/liver

35

Method Description

LDL/ MIC

MPL

Species/ Tissues

Reference

Tiamulin

Organic solvent extraction followed by GC of the 8-hydroxymutilin metabolite.

0.2 ppm

0.4 ppm

Swine/liver

35

Extracts confirmed by GC/MS

NE

0.4 ppm

Swine/liver

35

Tin

Tissue is dry ashed and dissolved and quantified by AAS (used to screen for organotin compounds)

0.02 ppm

NE

All/kidney, liver, muscle

1

Toxaphene

 Micro alumina assay: column chromatography plus GLC

0.50 ppm

NE

All/fat, pp

1

Mills method: Florisil column chromatography plus GLC

0.50 ppm

1.00 ppm

All/fat, pp

1

Trichlorfon

Tissue extracts are quantified by GLC with flame photometric or nitrogen-phosphorous flame ionization detector

NE

NE

All/liver, muscle

1

Tylosin

Antibiotic screen test (Swab): ability of tissue fluids containing anti- microbial activity to inhibit microbial growth

0.20 ppm

NE

All/kidney

Liquid-liquid extraction followed by HPLC-UV detection

0.1 ppm

NE

Cattle/muscle

14

Virginiamycin

Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity to inhibit microbial growth

0.64 ppm

NE

Swine/ kidney, liver, muscle

36

Organic solvent extraction followed by HPLC/UV quantification

0.1 ppm

NE

All/kidney, liver, muscle

1

Zeranol and metabolite taleranol

4.0 ppb (taleranol)

NE

Cattle, sheep

1

Zinc

2 ppb

NE

All/kidney, liver, muscle

1