Method Description

LDL/MIC

MPL

Species/Tissues

Ref.

Albendazole (amino sulfone metabolite)

Marker residue detected and quantified by HPLC- fluorescence detection

20 ppb

50 ppb

Cattle/liver Cattle/muscle Sheep/muscle

1

Extraction with organic solvents followed by HPLC with UV detection; confirmed by GC/EI/MS

0.05 ppm

NE

Red meat liver, muscle

1

Aldicarb and Metabolites

GPC plus HPLC with post- column fluorescence detection; extracts verified by oxidation to the sulfone

5 ppb

10 ppb

All/liver

1

Aldrin

Micro alumina assay: column chromatography plus GLC

0.02 ppm

NE

All/fat pp

1

GPC plus GLC

0.02 ppm

0.05 ppm

All/fat

1

Mills method: Florisil column chromatography plus GLC

0.02 ppm

0.10 ppm

All/fat pp

1

Extracts from GPC or Mills confirmed by GC/MS.

0.03 ppm

NE

All/fat,PP

1

Method Description

LDL/MIC

MPL

Species/Tissues

Ref.

Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity to inhibit microbial growth

0.02 ppm

0.02 ppm

Cattle, swine/ kidney,liver, muscle

2

0.01 ppm

0.01 ppm

Cattle, swine/ kidney,liver, muscle

1

Trihydrate

0.01 ppm

0.01 ppm

Cattle, swine/ all

3

Apramycin

Sample extraction TLC; bioautographed using Bacillus subtilis as a test organism

0.05 ppm

0.1 ppm

Swine/kidney ,muscle

1

Arsenate, Copper  Arsenate, Lead Arsenate, Magnesium Arsenate, Sodium Arsenic,Arsenite, Potassium Arsenite,

Dry ashed tissue dissolved and reacted to produce arsine gas, which reacts to form blue complex for colorimetric quantification

0.05 ppm

0.20 ppm

All/kidney, liver, muscle

1

Extracts confirmed by GC/MS

5 ppb

NE

All/fat

1

Bacitracin, zinc

Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity to inhibit microbial growth

0.05 ppm

NE

All/kidney, liver, muscle

4

Bambermycins

Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity to inhibit microbial growth

25 ppb

NE

All/kidney, liver, muscle,

5

Bendiocarb

GPC plus HPLC with post-column fluroescence detection.

5 ppb

10 ppb

All/liver

1

Method Description

LDL/MIC

MPL

Species/Tissues

Ref.

 Benomyl

pH extraction with organic solvents; followed by HPLC ppm with UV detection; extracts derivatizedand confirmed by GC/EI/MS

0.05 ppm

NE

Poultry/ liver, muscle

1

BHC

Micro alumina assay: column chromatography plus GLC

0.01 ppm

NE

All/fat,PP

1

GPC plus GLC

0.01 ppm

0.05 ppm

All/fat

1

Beta and delta isomers: GPC plus GLC

0.02 ppm

0.05 ppm

All/fat

1

Mills method: Florisil column chromatography plus GLC

0.02 ppm

0.10 ppm

All/fat, PP

1

Extracts from GPC or Mills confirmed by GC/MS

0.02 ppm

NE

All/fat, PP

1

Bufencarb

GPC plus HPLC with post- column fluorescence detection. Extracts are subjected to reverse phase chromatography, derivativized and confirmed by GC/MS

5 ppb

10 ppb

All/liver

1

Dry ashed tissue is dissolved and reacted to produce arsine gas, which reacts to form bluecomplex for colorimetric quantification

0.05 ppm

0.20 ppm

All/kidney, liver, muscle

6

Dry ashed tissue is dissolved and quantified by AAS or by inductively coupled plasma

0.5 ppd

NE

All/kidney , liver, muscle

1

Dry ashed tissue is quantified by anodic stripping voltammetry

1.0 ppb

NE

Poultry, kidney, liver

7

Wet ashed tissue is quantified by AAS

All

8

Extraction with organic solvents followed by HPLC with UV detection; extracts confirmed by GC/EI/MS

0.05 ppm

NE

Red meat/ liver, muscle, PP

1

GPC plus GLC

0.02 ppm

0.05 ppm

Red meat/fat

1

Extracts confirmed by GC/EI/MS

7.5 ppb

NE

Swine/ liver, muscle

1

Method Description

LDL/MIC

MPL

Species/Tissues

Ref.

Dry ashed tissue is dissolved and reacted to produce arsine gas, which reacts to form blue complex for colorimetric quantification

0.05 ppm

0.20 ppm

All/kidney, liver, muscle

9

 Carbaryl

Extracts are subjected to reverse phase chromatography, derivativized and confirmed by GC/MS

5 ppb

10 ppb

All/liver

1

Carbofuran and metabolite

Extracts are subjected to reverse phase chromatography, derivativized and confirmed by GC/MS

5 ppb

10 ppb

All/liver

1

Carbophenothion

Tissue extracts are quantified by GLC with flame photometric or nitrogen-phosphorous flame ionization detector

0.10 ppm

NE

All/liver, muscle

1

 Chloramphenicol

Tissue extracts are screened by E-Z screen

25 ppb

NE

Calf/ muscle, kidney

10

Tissue extract screened for chloramphenicol by CELIA CA

5ppb

NE

Calf/ muscle

11

Extraction of parent and glucuronide using C18 columnswith GC capillary quantification as the trimethylsilyl derivative

0.15 ppb

NE

Calf/muscle

1

Extracts are confirmed using NICI/MS

0.15 ppb

NE

Calf/muscle

1

C18 cleanup of the hydrolyzed extract with GC capillary quantification as the trimethyl derivative

5 ppb

NE

Calf/urine

1

Extracts are confirmed using NICI/MS

5 ppb

NE

Calf/urine

1

Chlordane

GPC followed by GC-ECD

0.10 ppm

0.30 ppm

All/fat

1

Mills method: Florisil column chromatography plus GLC

0.15 ppm

0.30 ppm

All/fat, PP

1

Extracts from GPC or Millsare confirmed by GC/MS

NE

NE

All/fat, PP

1

(Kepone)

0.03 ppm

0.20 ppm

All/fat

1

(cattle)

NE

Poultry, red meat/fat

1

Method Description

LDL/MIC

MPL

Species/Tissues

Ref.

(chlorfenvinphos)

GPC extracts are confirmed by CG/EI/MS

0.10 ppm (red meat)

NE

Poultry, red meat/fat

1

(stirophos)

GPC plus GLC

0.05 ppm

0.30 ppm

All/fat

1

0.10 ppm (cattle)

NE

Poultry, red meat/fat

1

Chlorpyrifos

GPC followed by GC-ECD

0.05 ppm

0.20 ppm

All/fat

1

0.50 ppm (swine)

NE

Poultry,swine/ fat

1

Chlortetracycline

Antibiotic screen test (Swab): ability of tissue fluids containing anti- microbial activity toinhibit microbial growth

0.01 ppm

NE

All/kidney

12

Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity to inhibit microbial growth 

0.01 ppm

NE

All/kidney liver, muscle

13

Extraction using C18 columns followed by HPLC with UV detection

0.15 ppm

0.25 ppm

All/kidney liver, muscle

1

Chromium

Dry ashed tissue is extracted with organic reagent and quantified using AAS

NE

NE

All/kidney liver, muscle

1

Clopidol

Organic solvent extraction with HPLC-UV detection

0.1

NE

Poultry/liver ppm

1

Organic solvent extraction with GC-EC detection

0.1 ppm

NE

Poultry/liver

1

 Clorsulon

Tissue extracts for HPLCare derivatized and confirmed by GC/MS

NE

Red meat/ kidney, muscle, liver, PP

1

Cloxacillin

Microbiological assay combined with HPLC separation andquantified by microbial inhibition

0.02 ppm

NE

Dairy cows/ kidney, liver, muscle

17

Cobalt

Dry ashed tissue is dissolved and quantified by AAS or by inductively coupled plasma

6 ppb

NE

All/kidney, liver, muscle

Method Description

LDL/MIC

MPL

Species/Tissues

Ref.

Copper

3 ppb

NE

All/kidney, liver, muscle

1

Coumaphos and oxygen analog

Tissue extracts are quantified by GLC with flame photometric or nitrogen-phosphorous flame ionization detector

0.10 ppm

NE

All/liver, muscle

1

GPC extracts are confirmed by CG/EI/MS

0.20 ppm

0.30 ppm

Red meat/fat

1

Cresylic acid

Tissue extracts are derivatized and determined by GC-EC

NE

NE

Poultry/fat

1

Crufomate (Ruelene)

Tissue extracts are quantified by GLC with flame photometric or nitrogen-phosphorous flame ionization detector

0.10 ppm

NE

All/liver, muscle

1

Cyanide salts

Aqueous extraction followed by a colori- metric determination

0.5 ppm

NE

All/all

1

For confirmation, cyanogen chloride is produced and determined by GC/EC

0.5 ppm

NE

All/all

1

[fenvalerate]

Organic solvent extracts are quantified as the sum of both isomers by GC/EC; extracts areconfirmed by GC/EI/MS

0.03 ppb

NE

All/fat

1

DDE (metabolites of DDT collectively reported as DDT)

GPC followed by GC-ECD

0.02 ppm

0.05 ppm

All/fat

1

Mills method: Florisil column chromatographyplus GLC

0.02 ppm

0.10 ppm

All/fat, PP

1

Extracts from GPC or Mills are confirmed by GC/MS (LRC)

0.02 ppm

NE

All/fat,PP

1

(isomers of DDT collectively reported as DDT)

GPC followed by GC-ECD

0.04 ppm

0.15 ppm

All/fat

1

Mills method:Florisil column chromatographyplus GLC

0.04 ppm

0.15 ppm

All/fat,PP

1

Decoquinate

Zymark Pytechnology System; organic solvent extraction followed by HPLC with fluorescence detection

0.20ppm

0.50 ppm

Cattle, poultry/ liver, muscle

14

Method Description

LDL/MIC

MPL

Species/Tissues

Ref.

Deltamethrin

Solvent extraction followed by a competitive ELISA determination

0.5 ppm

NE

All/fat

1

Diazinon

Tissue extracts are quantified by GLC with flame photometric or nitrogen-phosphorous flame ionization detector

0.1 ppm

NE

All/liver, muscle

1

Dibutyltin dilaurate

Tissue extraction acidhydrolysis-morin derivatization--HPLC-UV

0.25 ppm

NE

Turkey/liver

1

Dieldrin

GPC plus GLC

0.01 ppm

0.05 ppm

All/fat

1

Mills method: Florisil column chromatography

0.01 ppm

0.10 ppm

All/fat,PP

1

Extracts from GPC or Mills are confirmed by GC/MS

0.02 ppm

NE

All/fat,PP

1

Diethylstilbestrol (DES)

Solid phase extraction technique using an internal standard followed by methylsilation for GC/MS quantification and confirmation

0.1 ppb

0.2 ppb

Cattle, sheep liver, muscle

1

Dihydrostreptomycin

Antibiotic screen test (Swab): ability of tissue fluids containing anti-microbial activity to inhibit microbial growth

0.25 ppm

NE

All/kidney

12

Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity toinhibit microbial growth

0.25 ppm

NE

All/kidney, liver, muscle

18

3, 5-Dimethyl-4-(methylthio)phenyl methylcarbamate and metabolite

GPC plus HPLC with post- column fluorescence detection Extracts are subjected to reverse phase chromatography, derivatized and confirmedby GC/MS

5 ppb

10 ppb

All/liver

1

Dimetridazole and hydroxy metabolite

Tissue extracts from HPLC are confirmed by GC/NICI/MS

1.0 ppb

NE

Turkey, swine/ muscle

14

Dioxacarb

Extracts are subjected to reverse phase chromatography, derivativized and confirmed by GC/MS

5 ppb

10 ppb

All/liver

1

Dioxathion

Tissue extracts are quantified by GLC with flame photometric or nitrogen-phosphorous flame ionization detector

0.10 ppm

NE

All/liver, muscle

 Dodecachloro- octahydro-1,3,4- metheno-2H- cyclobuta(cd)- pentalene[Mirex]

Extracts from GPC or Mills are confirmed by GC/MS

0.05 ppm

NE

All/fat,PP

1

Method Description

LDL/MIC

MPL

Species/Tissues

Ref.

Endosulfan I

GPC extracts are confirmed by GC/EI/MS

0.02ppm

NE

Red meat/fat

1

Endosulfan II

GPC plus GLC

0.02ppm

Micro alumina assay: column chromatography plus GLC

0.03 ppm

NE

All/fat,PP

1

Mills method: Florisilcolumn chromatography plus GLC

0.03 ppm

0.10 ppm

All/fat, PP

1

Extracts from GPC or Mills are confirmed by GC/MS

0.05 ppm

NE

All/fat,PP

1

Erythromycin

Antibiotic screen test (Swab): ability of tissue fluids containing antimicrobial activity to inhibit microbial growth

25 ppb

NE

All/kidney

12

Microbiological assay procedure: ability of tissue fluids containing antimicrobial activity to inhibit microbial growth

25 ppb

NE

All/kidney, liver, muscle

19

Ethion and oxygen analog

Tissue extracts are quantified by GLC with flame photometric or nitrogen-phosphorous flame ionization detector

0.10 ppm

NE

All/liver, muscle

1

Tissue extracts are quantified by GLC with flame photometric or nitrogen-phosphorous flame ionization detector

NE

NE

All/liver muscle

1

Ethylene dibromide

Residue is co-distilled from aqueous suspension and quantified by GLC

0.5 ppb

1.0 ppb

All/fat

1

MS by NICI to determine bromine

1 ppb

NE

All/fat

1

Fenbendazole

Extraction with organic solvents followed by HPLC with UV detection; extracts derivatized and confirmed by GC/EI/MS

0.10 ppm

NE

Red meat/ liver, muscle

1

Tissue extracts are quantifiedby HPLC

200 ppb

400 ppb

Cattle, calf/ liver

20

Quantification extract purified by TLC, derivatized and identified by HPLC fluorescence

200 ppb

NE

Cattle, calf/ liver

20

Method Description

LDL/MIC

MPL

Species/Tissues

Ref.

Fenitrothion

Tissue extracts are quantified by GLC with flame photometric or nitrogen- phosphorous flame ionization detector

0.10 ppm

NE

All/liver, muscle

1

Fenpropathrin

Solvent extraction followed by a competitive ELISA determination

1.0 ppm

NE

All/fat

14

Flucythrinate

Organic solvent extracts are quantified as the sum of both isomers by GC/EC; extracts are confirmed by GC/EI/MS

1 ppm

NE

All/fat

1

Solvent extraction followed by a competitive ELISA determination

1.0 ppm

NE

All/fat

1

Gasoline

 Fat from product is heated in a sealed vial and gasoline components are identified by pattern recognition using GC/flame ionization detection

0.1 ppm

NE

All/muscle

1

Gentamicin sulfate

Microbiological assay procedure: ability of tissue extracts containing antimicrobial activity to inhibit microbial growth

NE

NE

Swine/kidney

22

Extraction followed by detection by HPLC with fluorescence detector

0.2 ppm

0.4 ppm

Swine/kidney

1

Halofuginone

Tissue extracts are quantified by HPLC-UV

0.05 ppm

0.05 ppm

Chicken/liver, muscle

1

Tissue extracts are confirmed by GC/MS/MS

0.05 ppm

NE

Chicken/liver, muscle

23